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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Observations on catalysis by hammerhead ribozymes are consistent with a two-divalent-metal-ion mechanism
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Observations on catalysis by hammerhead ribozymes are consistent with a two-divalent-metal-ion mechanism

机译：锤头状核酶催化的观察结果与二价金属离子机理一致

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摘要

Significant cleavage by hammerhead ri- bozymes requires activation by divalent metal ions. Several models have been proposed to account for the influence of metal ions on hammerhead activity. A number of recent papers have presented data that have been interpreted as supporting a one-metal-hydroxide-ion mechanism. In addi- tion, a solvent deuterium isotope effect has been taken as evidence against a proton transfer in the rate-limiting step of the cleavage reaction. We propose that these data are more easily explained by a two-metal-ion mechanism that does not involve a metal hydroxide, but does involve a proton transfer in the rate-limiting step.
机译：锤头状核酶的显着裂解需要被二价金属离子激活。已经提出了几种模型来说明金属离子对锤头活动的影响。许多最近的论文已经提出了被解释为支持一种金属氢氧根离子机理的数据。此外，在裂解反应的限速步骤中，溶剂氘同位素效应已被证明可防止质子转移。我们提出，这些数据更容易通过不涉及金属氢氧化物但涉及限速步骤中质子转移的双金属离子机理来解释。

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