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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >VARIABLE EXPRESSION OF MYOGLOBIN AMONG THE HEMOGLOBINLESS ANTARCTIC ICEFISHES
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VARIABLE EXPRESSION OF MYOGLOBIN AMONG THE HEMOGLOBINLESS ANTARCTIC ICEFISHES

机译：血红蛋白性无极结节中的肌红蛋白的可变表达

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The important intracellular oxygen-binding protein, myoglobin (Mb), is thought to be absent from oxidative muscle tissues of the family of hemoglobinless Antarctic icefishes, Channichthyidac. Within this family of fishes, which is endemic to the Southern Ocean surrounding Antarctica, there exist 15 known species and II genera, To date, we have examined eight species of icefish (representing seven genera) using immunoblot analyses. Results indicate that Mb is present in heart ventricles from five of these species of icefish, Mb is absent from heart auricle and oxidative skeletal muscle of all species, We have identified a 0.9-kb mRNA in Mb-expressing species that hybridizes with a Mb cDNA probe from the closely related red-blooded Antarctic nototheniid fish, Notothenia coriiceps. in confirmation that the 0.9-kb mRNA encodes Mb, we report the full-length Mb cDNA sequence of the ocellated icefish, Chionodraco rastrospinosus. Of the eight icefish species examined, three lack Mb polypeptide in heart ventricle, although one of these expresses the Mb mRNA, All species of icefish retain the Mb gene in their genomic DNA, Based on phylogeny of the icefishes, loss of Mb expression has occurred independently at least three times and by at least two distinct molecular mechanisms during speciation of the family. [References: 31]
机译：重要的细胞内氧结合蛋白，肌红蛋白（Mb）被认为是无血红蛋白的南极冰鱼类Channichthyidac家族的氧化性肌肉组织所缺少的。在这个南极洲南部海洋特有的鱼类家族中，存在15个已知物种和II属。迄今为止，我们已经使用免疫印迹分析检查了8种冰鱼（代表7个属）。结果表明，这些鱼类中的五个物种的心室中均存在Mb，所有物种的心耳和氧化性骨骼肌中均不存在Mb。我们已在与Mb cDNA杂交的Mb表达物种中鉴定出0.9kb的mRNA。密切相关的南极红血统拟南芥鱼类Notothenia coriiceps的探针。为了确认0.9-kb mRNA编码Mb，我们报道了有卵冰鱼Chionodraco rastrospinosus的全长Mb cDNA序列。在检查的8种冰鱼物种中，有3种在心室中缺乏Mb多肽，尽管其中一种表达Mb mRNA。所有种类的冰鱼都在其基因组DNA中保留了Mb基因。基于冰鱼的系统发育，已经发生了Mb表达缺失在物种形成过程中至少独立地发生了至少三次，并通过至少两种不同的分子机制。 [参考：31]

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