A general purpose RNA-cleaving DNA enzyme

Stephen W. Santoro; Gerald F. Joyce

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A general purpose RNA-cleaving DNA enzyme

【24h】

A general purpose RNA-cleaving DNA enzyme

机译：通用的RNA切割DNA酶

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

An in vitro selection procedure was used to develop a DNA enzyme that can be made to cleave almost any targeted RNA substrate under simulated physiological con- ditions. The enzyme is comprised of a catalytic domain of 15 deoxynucleotides, flanked by two substrate-recognition do- mains of seven to eight deoxynucleotides each. The RNA substrate is bound through Watson-Crick base pairing and is cleaved at a particular phosphodiester located between an unpaired purine and a paired pyrimidine residue.

机译：使用体外选择程序开发了一种DNA酶，该酶可以在模拟的生理条件下裂解几乎所有靶向的RNA底物。该酶由15个脱氧核苷酸的催化结构域组成，两侧是两个底物识别域，每个域有7至8个脱氧核苷酸。 RNA底物通过沃森-克里克碱基配对结合，并在未配对的嘌呤和配对的嘧啶残基之间的特定磷酸二酯处裂解。

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第9期|p.4262-4266|共5页
作者
Stephen W. Santoro; Gerald F. Joyce;
展开▼
作者单位

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词

相似文献

外文文献
中文文献
专利

1. Potent inhibition of HIV-1 gene expression and TAT-mediated apoptosis in human T cells by novel mono- and multitarget anti-TAT/Rev/Env ribozymes and a general purpose RNA-cleaving DNA-enzyme. [J] . Unwalla H, Chakraborti S, Sood V, Antiviral Research . 2006,第2期

机译：新型单和多靶点抗TAT / Rev / Env核酶和通用的RNA裂解DNA酶可有效抑制人T细胞中HIV-1基因表达和TAT介导的细胞凋亡。
2. Targeted delivery of RNA-cleaving DNA enzyme (DNAzyme) to tumor tissue by transferrin-modified, cyclodextrin-based particles. [J] . Pun SH, Tack F, Bellocq NC, Cancer biology & therapy . 2004,第7期

机译：通过转铁蛋白修饰的，基于环糊精的颗粒靶向性地将裂解RNA的DNA酶（DNAzyme）递送至肿瘤组织。
3. Suppression of Hepatitis C Virus Genome Replication in Cells with RNA-Cleaving DNA Enzymes and Short-Hairpin RNA [J] . Lee Bokhui, Kim Kyung Bo, Oh Sangtaek, Oligonucleotides . 2010,第6期

机译：RNA裂解DNA酶和短发RNA抑制细胞中的丙型肝炎病毒基因组复制。
4. DNA Logic Circuits with a DNA Polymerase and a Nicking Enzyme [C] . Ryo Hirose, Satoshi Kobayashi, Ken Komiya International conference on parallel and distributed processing techniques and applications;PDPTA 2011 . 2012

机译：具有DNA聚合酶和切刻酶的DNA逻辑电路
5. Selection and analysis of RNA-cleaving deoxyribozymes and ribozymes: Application toward understanding enzyme structure and function. [D] . Emilsson, Gail Annette Mitchell. 2003

机译：RNA切割脱氧核糖酶和核酶的选择和分析：在理解酶结构和功能中的应用。
6. A general purpose RNA-cleaving DNA enzyme [O] . Stephen W. Santoro, Gerald F. Joyce 1997

机译：通用的RNA切割DNA酶
7. Targeted delivery of RNA-cleaving DNA enzyme (DNAzyme) to tumor tissue by transferrin-modified, cyclodextrin-based particles [O] . Suzie H. Pun, Frederik Tack, Nathalie C. Bellocq, 2004

机译：通过转铁蛋白改性的环糊精基颗粒靶向RNA切割的DNA酶（DNAzyme）至肿瘤组织

A general purpose RNA-cleaving DNA enzyme

摘要

著录项

相似文献

相关主题

期刊订阅