A new reporter cell line to monitor HIV infection and drug susceptibility in vitro

Alain Gervaix; Daniel West; Lorenzo M. Leoni

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A new reporter cell line to monitor HIV infection and drug susceptibility in vitro

机译：新型记者细胞系可在体外监测HIV感染和药物敏感性

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Determination of HIV infectivity in vitro and its inhibition by antiretroviral drugs by monitoring reduction of production of p24 antigen is expensive and time consuming. Such assays also do not allow accurate quantitation of the number of infected cells over time. To develop a simple, rapid, and direct method for monitoring HIV infection, we generated a stable T-cell line (CEM) containing a plasmid encoding the green fluorescent protein (humanized S65T GFP) driven by the HIV-1 long terminal repeat.

机译：通过监测p24抗原产生的减少来确定HIV的体外感染性及其在抗逆转录病毒药物中的抑制作用既昂贵又费时。这样的测定还不允许随时间准确定量感染细胞的数量。为了开发一种简单，快速，直接的方法来监测HIV感染，我们生成了稳定的T细胞系（CEM），其中包含编码由HIV-1长末端重复序列驱动的绿色荧光蛋白（人源化S65T GFP）的质粒。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第9期|p.4653-4658|共6页
作者
Alain Gervaix; Daniel West; Lorenzo M. Leoni;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. A cell line that secretes inducibly a reporter protein for monitoring herpes simplex virus infection and drug susceptibility. [J] . Wang YC, Kao CL, Liu WT, Journal of Medical Virology . 2002,第4期

机译：一种细胞系，可诱导性地分泌一种报告蛋白，用于监测单纯疱疹病毒感染和药物敏感性。
2. Human immunodeficiency virus (HIV) type 1 infection status and in vitro susceptibility to HIV infection among high-risk HIV-1-seronegative hemophiliacs. [J] . Lederman MM, Jackson JB, Kroner BL, The Journal of Infectious Diseases . 1995,第1期

机译：高危HIV-1血清阴性血友病患者中1型人类免疫缺陷病毒（HIV）感染状况和体外HIV易感性。
3. Fetal immune activation to malaria antigens enhances susceptibility to in vitro HIV infection in cord blood mononuclear cells. [J] . Steiner K, Myrie L, Malhotra I, The Journal of Infectious Diseases . 2010,第6期

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4. Targeted femtosecond laser driven drug delivery within HIV-1 infected cells: In-vitro studies [C] . Charles Maphanga, Saturnin Ombinda-Lemboumba, Sello Manoto, Optical interactions with tissue and cells XXVIII . 2017

机译：靶向飞秒激光驱动的HIV-1感染细胞内药物递送：体外研究
5. In-vitro T cell death induced by Aldrithiol-inactivated HIV virions as a model for T cell homeostasis failure in people with HIV-1 infection [D] . Kibirige-Shacklett, Catherine N. 2009

机译：Aldrithiol灭活的HIV病毒粒子诱导的体外T细胞死亡是HIV-1感染者T细胞稳态失败的模型
6. A new reporter cell line to monitor HIV infection and drug susceptibility in vitro [O] . Alain Gervaix, Daniel West, Lorenzo M. Leoni, 1997

机译：新的记者细胞系可监测体外的HIV感染和药物敏感性
7. Dendritic Cells from HIV Controllers Have Low Susceptibility to HIV-1 Infection In Vitro but High Capacity to Capture HIV-1 Particles [O] . Hamimi, Chiraz, David, Annie, Versmisse, Pierre, 2016

机译：HIV控制者的树突状细胞体外对HIV-1感染的敏感性较低，但捕获HIV-1颗粒的能力却很高

A new reporter cell line to monitor HIV infection and drug susceptibility in vitro

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