Modulation of glucose responsiveness of insulinoma β-cells by graded overexpression of glucokinase

Haiyan Wang; Patrick B. Iynedjian

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Modulation of glucose responsiveness of insulinoma β-cells by graded overexpression of glucokinase

机译：分级过表达葡萄糖激酶对胰岛素瘤β细胞葡萄糖反应性的调节

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Insulinoma β-cells capable of overexpressing glucokinase under the control of a doxycycline-dependent transcriptional transactivator were established from parental INS-1 cells. Glucokinase could be maximally induced to a level more than 20 times the basal level after 36 h of culture with doxycycline. Intermediate levels of induction could be achieved by varying doses of, and time of culture with, the inducer. The rate of glycolysis was measured in cells with 3-, 5-, and 8-fold increment in glucokinase activity above the noninduced level. Proportionate increases in glycolytic flux occurred in cells cultured at low physiological glucose con- centration.

机译：由亲本INS-1细胞建立了在强力霉素依赖性转录反式激活因子的控制下能够过表达葡萄糖激酶的胰岛素瘤β细胞。用强力霉素培养36小时后，可最大程度地诱导葡萄糖激酶的水平超过基础水平的20倍。通过改变诱导剂的剂量和培养时间可以达到中等水平的诱导。测量了葡萄糖激酶活性在非诱导水平以上3、5和8倍增加的细胞中的糖酵解速率。在低生理葡萄糖浓度下培养的细胞中发生的糖酵解通量成比例增加。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第9期|p.4372-4377|共6页
作者
Haiyan Wang; Patrick B. Iynedjian;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. Glucokinase is an integral component of the insulin granules in glucose-responsive insulin secretory cells and does not translocate during glucose stimulation. [J] . Arden C, Harbottle A, Baltrusch S, Diabetes . 2004,第9期

机译：葡萄糖激酶是葡萄糖反应性胰岛素分泌细胞中胰岛素颗粒的组成部分，在葡萄糖刺激过程中不会移位。
2. Modulation of glucokinase by glucose, small-molecule activator and glucokinase regulatory protein: steady-state kinetic and cell-based analysis [J] . Bourbonais Francis J., Chen Jing, Huang Cong, The Biochemical Journal . 2012,第3aPta3期

机译：葡萄糖，小分子激活剂和葡萄糖激酶调节蛋白对葡萄糖激酶的调节：稳态动力学和基于细胞的分析
3. Modulation of glucokinase by glucose, small-molecule activator and glucokinase regulatory protein: steady-state kinetic and cell-based analysis [J] . Cong Huang, Francis?J. Bourbonais, James?A. Landro, The biochemical journal . 2012,第3期

机译：葡萄糖，小分子激活剂和葡萄糖激酶调节蛋白对葡萄糖激酶的调节：稳态动力学和基于细胞的分析
4. Lys169 of Human Glucokinase Is a Determinant for Glucose Phosphorylation: Implication for the Atomic Mechanism of Glucokinase Catalysis [C] . Jian Zhang, Chenjing Li, Ting Shi, ICMSI;International conference of molecular simulations and applied informatics technologies . 2010

机译：人葡萄糖激酶的Lys169是葡萄糖磷酸化的决定因素：葡萄糖激酶催化的原子机理的含义。
5. Insulin responsiveness of the glucose transporter 4 in 3T3-L1 cells depends on the presence of sortilin. [D] . Buckler-Pena, Dana. 2014

机译：葡萄糖转运蛋白4在3T3-L1细胞中的胰岛素反应性取决于sortilin的存在。
6. Modulation of glucose responsiveness of insulinoma β-cells by graded overexpression of glucokinase [O] . Haiyan Wang, Patrick B. Iynedjian 1997

机译：分级过表达葡萄糖激酶对胰岛素瘤β细胞葡萄糖反应性的调节
7. Modulation of glucose responsiveness of insulinoma -cells by graded overexpression of glucokinase [O] . H. Wang, P. B. Iynedjian 1997

机译：葡萄糖酮血磷酶渐进过表达的胰岛素瘤 - 细胞葡萄糖反应的调节

Modulation of glucose responsiveness of insulinoma β-cells by graded overexpression of glucokinase

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