Crystal structure of glutamate-1-semialdehyde aminomutase: An α _2-dimeric vitamin B_6-dependent enzyme with asymmetry in structure and active site reactivity

Michael Hennig; Bernhard Grimm; Roberto Contestabile

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Crystal structure of glutamate-1-semialdehyde aminomutase: An α _2-dimeric vitamin B_6-dependent enzyme with asymmetry in structure and active site reactivity

机译：谷氨酸-1-半醛氨基变位酶的晶体结构：α_2-二聚体维生素B_6依赖性酶，结构和活性位点反应性均不对称

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The three-dimensional structure of gluta- mate-1-semialdehyde aminomutase (EC 5.4.3.8), an α_2- dimeric enzyme from Synechococcus, has been determined by x-ray crystallography using heavy atom derivative phasing. The structure, refined at 2.4-A deg resolution to an R-factor of 18.7/100 and good stereochemistry, explains many of the en- zyme's unusual specificity and functional properties. The overall fold is that of aspartate aminotransferase and related B6 enzymes, but it also has specific features. The structure of the complex with gabaculine, a substrate analogue, shows unexpectedly that the substrate binding site involves residues from the N-terminal domain of the molecule, notably Arg-32.

机译：谷氨酸-1-半醛氨基变位酶（EC 5.4.3.8）的三维结构（Synechococcus的一种α_2-二聚酶）已通过X射线晶体学使用重原子衍生物定相法确定。该结构以2.4A deg的分辨率精炼到18.7 / 100的R因子和良好的立体化学，解释了许多酶的不同寻常的特异性和功能特性。整体折叠是天冬氨酸转氨酶和相关的B6酶的折叠，但它也具有特定的功能。与加巴奎林（一种底物类似物）形成的复合物的结构出乎意料地表明，底物结合位点涉及分子N末端结构域的残基，尤其是Arg-32。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第10期|p.4866-4871|共6页
作者
Michael Hennig; Bernhard Grimm; Roberto Contestabile;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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6. Crystal structure of glutamate-1-semialdehyde aminomutase: An α2-dimeric vitamin B6-dependent enzyme with asymmetry in structure and active site reactivity [O] . Michael Hennig, Bernhard Grimm, Roberto Contestabile, 1997

机译：谷氨酸-1-半醛氨基变位酶的晶体结构：α2-二聚体维生素B6依赖性酶结构和活性位点反应均不对称
7. Crystal structure of glutamate-1-semialdehyde. An alpha-2-dimeric vitamin B6 dependent enzyme with asymmetry in structure and active site reactivity [O] . HENNIG M., GRIMM B., R. CONTESTABILE, 1997

机译：谷氨酸-1-半醛的晶体结构。具有结构和活性部位反应性不对称的α-2-二聚维生素B6依赖性酶

Crystal structure of glutamate-1-semialdehyde aminomutase: An α _2-dimeric vitamin B_6-dependent enzyme with asymmetry in structure and active site reactivity

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