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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Early embryonic lethality caused by targeted disruption of the mouse selenocysteine tRNA gene (Trsp)
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Early embryonic lethality caused by targeted disruption of the mouse selenocysteine tRNA gene (Trsp)

机译：小鼠硒代半胱氨酸tRNA基因（Trsp）的定向破坏引起的早期胚胎致死率

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摘要

Selenoprotein biosynthesis is mediated by tRNA~Sec, which inserts selenocysteine at UGA codons in a complex, context-specific manner. This opal suppressor serves in the conversion of serine to selenocysteine as well. The mouse tRNA~Sec gene (Trsp) maps to a proximal segment of chromosome 7. We constructed mice carrying a targeted deletion of the Trsp gene. The heterozygous mutants were viable, fertile, and appeared normal. Although the level of tRNA~Sec was reduced to about 50/100-80/100 of the wild type in most organs, one of the selenoproteins, glutathione peroxi- dase, remained unaffected in the levels of its mRNA, protein, and enzyme activity, indicating that the haploid amount of tRNA~Sec is not limiting in its biosynthesis.
机译：硒蛋白的生物合成是由tRNA〜Sec介导的，它以一种复杂的，特定于上下文的方式在UGA密码子上插入了硒代半胱氨酸。该蛋白石抑制剂也可将丝氨酸转化为硒代半胱氨酸。小鼠tRNA〜Sec基因（Trsp）定位到7号染色体的近端片段。我们构建了带有Trsp基因靶向缺失的小鼠。杂合突变体是活的，可育的，并且看起来是正常的。尽管在大多数器官中，tRNA〜Sec的水平已降至野生型的50 / 100-80 / 100，但其中一种硒蛋白谷胱甘肽过氧化物酶仍不受其mRNA，蛋白质和酶活性的影响。 ，表明tRNA〜Sec的单倍体数量没有限制其生物合成。

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