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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Engineering subunit association of multisubunit proteins: A dimeric streptavidin
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Engineering subunit association of multisubunit proteins: A dimeric streptavidin

机译：多亚基蛋白的工程亚基缔合：二聚链霉亲和素

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摘要

A dimeric streptavidin has been designed by molecular modeling using effective binding free energy cal- culations that decompose the binding free energy into elec- trostatic, desolvation, and side chain entropy loss terms. A histidine-127 - aspartic acid (H127D) mutation was suffi- cient to introduce electrostatic repulsion between subunits that prevents the formation of the natural tetramer. However, the high hydrophobicity of the dimer-dimer interface, which would be exposed to solvent in a dimeric streptavidin, suggests that the resulting molecule would have very low solubility in aqueous media.
机译：通过有效的结合自由能计算，通过分子建模设计了二聚链霉亲和素，该结合能将结合自由能分解为静电，去溶剂化和侧链熵损失项。组氨酸127-天冬氨酸（H127D）突变足以在亚基之间引入静电排斥，从而阻止了天然四聚体的形成。然而，二聚体-二聚体界面的高疏水性将暴露于二聚链霉亲和素中的溶剂，这表明所得分子在水性介质中的溶解度非常低。

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