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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Assembly and positioning of microtubule asters in microfabricated chambers
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Assembly and positioning of microtubule asters in microfabricated chambers

机译:在微加工室中装配和定位微管紫苑

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Intracellular organization depends on a va- riety of molecular assembly processes; while some of these have been studied in simplified cell-free systems, others depend on the confined geometry of cells and cannot be reconstructed using bulk techniques. To study the latter processes in vitro, we fabricated microscopic chambers that simulate the closed environment of cells. We used these chambers to study the positioning of microtubule asters. Microtubule assembly alone, without the action of molecular motors, is sufficient to position asters.
机译:细胞内的组织取决于各种分子组装过程。尽管其中一些已在简化的无细胞系统中进行了研究,但其他一些则取决于有限的细胞几何结构,无法使用批量技术进行重建。为了在体外研究后者的过程,我们制造了模拟细胞封闭环境的微观腔室。我们使用这些小室来研究微管紫苑的定位。无需分子马达的作用,仅微管组件就足以定位紫position。

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