Targeted gene expression without a tissue-specific promoter: Creating mosaic embryos using laser-induced single-cell heat shock

Marc S. Halfon; Hiroyuki Kose; Akira Chiba

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Targeted gene expression without a tissue-specific promoter: Creating mosaic embryos using laser-induced single-cell heat shock

【24h】

Targeted gene expression without a tissue-specific promoter: Creating mosaic embryos using laser-induced single-cell heat shock

机译：没有组织特异性启动子的靶向基因表达：使用激光诱导的单细胞热休克创建镶嵌胚

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We have developed a method to target gene expression in the Drosophila embryo to a specific cell without having a promoter that directs expression in that particular cell. Using a digitally enhanced imaging system to identify single cells within the living embryo, we apply a heat shock to each cell individually by using a laser microbeam. A 1- to 2-min laser treatment is sufficient to induce a heat-shock response but is not lethal to the heat-shocked cells.

机译：我们已经开发出一种方法，可以将果蝇胚胎中的基因表达靶向特定的细胞，而无需具有指导该特定细胞中表达的启动子。通过使用数字增强型成像系统识别活胚中的单个细胞，我们通过使用激光微束分别对每个细胞施加热激。 1至2分钟的激光处理足以引起热激反应，但对热激细胞没有致死性。

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第12期|p.6255-6260|共6页
作者
Marc S. Halfon; Hiroyuki Kose; Akira Chiba;
展开▼
作者单位

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词

相似文献

外文文献
中文文献
专利

1. Soybean actin, heat shock protein, and ribosomal protein promoters direct tissue-specific transgene expression in transgenic soybean [J] . Zhang Zhifen, Finer John J. In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association . 2015,第1期

机译：大豆肌动蛋白，热休克蛋白和核糖体蛋白启动子指导转基因大豆中组织特异性转基因的表达
2. Temporal and spatial manipulation of gene expression in Xenopus embryos by injection of heat shock promoter-containing plasmids. [J] . Michiue T, Asashima M Developmental dynamics: an official publication of the American Association of Anatomists . 2005,第2期

机译：通过注射含有热激启动子的质粒，对非洲爪蟾胚胎中的基因表达进行时空操纵。
3. The Natural Compound Cantharidin Induces Cancer Cell Death through Inhibition of Heat Shock Protein 70 (HSP70) and Bcl-2-associated Athanogene Domain 3 (BAG3) Expression by Blocking Heat Shock Factor 1 (HSF1) Binding to Promoters [J] . Joo Ae Kim, Youngmi Kim, Byoung-Mog Kwon, The Journal of biological chemistry . 2013,第40期

机译：天然化合物赤素蛋白通过阻塞热休克因子1（HSF1）与启动子结合来抑制热休克蛋白70（HSP70）和Bcl-2相关的邻苯基结构域3（BAG3）表达来诱导癌细胞死亡
4. USING SIMPLE RULES ON PRESENCE AND POSITIONING OF MOTIFS FOR PROMOTER STRUCTURE MODELING AND TISSUE-SPECIFIC EXPRESSION PREDICTION [C] . ALEXIS VANDENBON, KENTA NAKAI International Conference on Genome Informatics . 2008

机译：用简单的规则关于启动子结构建模和组织特异性表达预测的基序的存在和定位
5. Low frequency electromagnetic field induction of heat shock gene expression: Promoter for targeted gene therapy. [D] . Frisch, Paul H. 2008

机译：低频电磁场诱导的热激基因表达：靶向基因治疗的启动子。
6. Targeted gene expression without a tissue-specific promoter: Creating mosaic embryos using laser-induced single-cell heat shock [O] . Marc S. Halfon, Hiroyuki Kose, Akira Chiba, 1997

机译：没有组织特异性启动子的靶向基因表达：利用激光诱导的单细胞热休克创建镶嵌胚
7. Temporal and spatial manipulation of gene expression inXenopus embryos by injection of heat shock promoter-containing plasmids [O] . Tatsuo Michiue, Makoto Asashima 2005

机译：注射含热休克启动子质粒基因表达胚胎的时间和空间操纵

Targeted gene expression without a tissue-specific promoter: Creating mosaic embryos using laser-induced single-cell heat shock

摘要

著录项

相似文献

相关主题

期刊订阅