The first step of aminoacylation at the atomic level in histidyl-tRNA synthetase

John G. Arnez; John G. Augustine; Dino Moras

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The first step of aminoacylation at the atomic level in histidyl-tRNA synthetase

机译：组氨酸-tRNA合成酶在原子水平上进行氨酰化的第一步

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The crystal structure of an enzyme- substrate complex with histidyl-tRNA synthetase from Esch- erichia coli, ATP, and the amino acid analog histidinol is described and compared with the previously obtained en- zyme-product complex with histidyl-adenylate. An active site arginine, Arg-259, unique to all histidyl-tRNA synthetases, plays the role of the catalytic magnesium ion seen in seryl- tRNA synthetase. When Arg-259 is substituted with histidine, the apparent second order rate constant (k_cat/K_m) for the pyrophosphate exchange reaction and the aminoacylation reaction decreases 1,000-fold and 500-fold, respectively.

机译：描述了一种酶-底物复合物的晶体结构，该酶-底物复合物具有来自大肠杆菌，ATP和氨基酸类似物组氨醇的组氨酸-tRNA合成酶，并与先前获得的具有组氨酸-腺苷酸的酶-产物复合物进行了比较。所有组氨酸-tRNA合成酶都具有的活性位点精氨酸Arg-259发挥了在丝氨酰tRNA合成酶中所见的催化镁离子的作用。当用组氨酸取代Arg-259时，焦磷酸盐交换反应和氨基酰化反应的表观二阶速率常数（k_cat / K_m）分别降低1000倍和500倍。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第14期|p.7144-7149|共6页
作者
John G. Arnez; John G. Augustine; Dino Moras;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. Aminoacylation Reaction in the Histidyl-tRNA Synthetase: Fidelity Mechanism of the Activation Step [J] . S. Dutta Banik, N. Nandi The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical . 2010,第6期

机译：组氨酸-tRNA合成酶中的氨基酰化反应：激活步骤的保真机理
2. Oxidative stress-induced modifications of histidyl-tRNA synthetase affect its tRNA aminoacylation activity but not its immunoreactivity. [J] . van Dooren SH, Raijmakers R, Pluk H, Biochemistry and Cell Biology . 2011,第6期

机译：氧化应激诱导的组氨酸-tRNA合成酶修饰会影响其tRNA氨酰化活性，但不会影响其免疫反应性。
3. Recognition of G-1:C73 Atomic Groups by Escherichia coli Histidyl-tRNA Synthetase [J] . Abbey E. Rosen, Karin Musier-Forsyth Journal of the American Chemical Society . 2004,第1期

机译：大肠杆菌组氨酸-tRNA合成酶对G-1：C73原子团的识别
4. An atomic-level insight into the surface step edge of GaN [C] . Kieu My Bui, Jun-Ichi Iwata, Mauro Boero, 応用物理学会秋季学術講演会;応用物理学会 . -1

机译：原子级洞察GaN的表面台阶边缘
5. Part I: Spectroscopic characterization of sol-gel-derived materials and dendritic compounds amenable to optical chemical sensor development. Part II: Steps towards the development of general aminoacylation of transfer RNA molecules via RNA-based catalysis: On-column synthesis and subsequent purification in a multiple turnover system. [D] . Bonzagni, Neil J. 2002

机译：第一部分：溶胶-凝胶衍生的材料和适合光学化学传感器开发的树枝状化合物的光谱表征。第二部分：通过基于RNA的催化作用，对转移RNA分子进行一般氨基酰化的步骤：柱上合成以及随后在多周转系统中的纯化。
6. The first step of aminoacylation at the atomic level in histidyl-tRNA synthetase [O] . John G. Arnez, John G. Augustine, Dino Moras, 1997

机译：组氨酸tRNA合成酶在原子水平上氨酰化的第一步
7. Recognition of tRNALeu by Aquifex aeolicus leucyl-tRNA synthetase during the aminoacylation and editing steps [O] . Yao, Peng, Zhu, Bin, Jaeger, Sophie, 2008

机译：在氨酰化和编辑步骤中通过水产阿古斯油杆菌亮氨酰-tRNA合成酶识别tRNALeu

The first step of aminoacylation at the atomic level in histidyl-tRNA synthetase

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