A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: Purification, gene cloning, and trans-Golgi localization

Kanwarpal S. Dhugga; Suresh C. Tiwari; Peter M. Ray

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: Purification, gene cloning, and trans-Golgi localization

【24h】

A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: Purification, gene cloning, and trans-Golgi localization

机译：一种可能与植物细胞壁合成有关的可逆糖基化多肽（RGP1）：纯化，基因克隆和反高尔基体定位

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We purified from pea (Pisum sativum) tissue an ≈40 kDa reversibly glycosylated polypeptide (RGP1) that can be glycosylated by UDP-Glc, UDP-Xyl, or UDP-Gal, and isolated a cDNA encoding it, apparently derived from a single-copy gene (Rgp1). Its predicted translation product has 364 aminoacyl residues and molecular mass of 41.5 kDa. RGP1 appears to be a membrane-peripheral protein. Immunogold labeling localizes it specifically to trans-Golgi dictyosomal cisternae. Along with other evidence, this suggests that RGP1 is involved in synthesis of xyloglucan and possibly other hemicelluloses.

机译：我们从豌豆（Pisum sativum）组织中纯化了一个大约40 kDa的可逆糖基化多肽（RGP1），该多肽可被UDP-Glc，UDP-Xyl或UDP-Gal糖基化，并分离出一种编码它的cDNA，该cDNA显然来自于复制基因（Rgp1）。它的预测翻译产物具有364个氨酰基残基，分子量为41.5 kDa。 RGP1似乎是膜外围蛋白。免疫金标记专门将其定位在反式高尔基体双体池中。与其他证据一起，这表明RGP1参与了木葡聚糖和其他半纤维素的合成。

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第14期|p.7679-7684|共6页
作者
Kanwarpal S. Dhugga; Suresh C. Tiwari; Peter M. Ray;
展开▼
作者单位

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词

相似文献

外文文献
中文文献
专利

1. Are the reversibly glycosylated polypeptides implicated in plant cell wall biosynthesis non-processive #beta#-glycosyltransferases? [J] . Inder M. Saxena, R. Malcolum Brown Jr Trends in Plant Science . 1999,第1期

机译：植物细胞壁生物合成中涉及的可逆糖基化多肽是否是非加工性的＃beta＃-糖基转移酶？
2. Purification, Characterization and Localization of Reversibly Glycosylated Polypeptide 1 from Rape (Brassica napus) Bee Pollen [J] . Qi Zhang, Tian Sun, Qingqing Liu, International Journal of Agriculture and Biology . 2019,第2期

机译：油菜蜂花粉中可逆糖基化多肽1的纯化，鉴定和定位
3. PCR-amplification and cloning of the coding region of a cDNA for a reversibly glycosylated polypeptide from rice with possible involvement in the biosynthesis of glucans [J] . Promila Gupta, Saurabh Raghuvanshi, Akhilesh K. Tyagi Journal of Plant Biochemistry and Biotechnology . 2000,第2期

机译：水稻中可逆糖基化多肽的cDNA编码区的PCR扩增和克隆，可能参与了葡聚糖的生物合成
4. CLONING, OVEREXPRESSION AND PURIFICATION OF THE POLYPEPTIDES SUBUNITS OF THE CYTOCHROME b_(559) FROM PLANTS [C] . Rafael Picorel, Maria Angeles Lujan, Miguel Alfonso, International Congress of Photosynthesis . 2005

机译：来自植物的细胞色素B_（559）的多肽亚基的克隆，过度表达和纯化
5. The Identification of Novel Genes Involved in the Synthesis, Secretion and Modification of Cell Wall Components in the Seed Coat of Arabidopsis thaliana. [D] . Arsovski, Andrej Adam. 2010

机译：拟南芥种皮中细胞壁成分合成，分泌和修饰的新基因的鉴定。
6. A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: Purification gene cloning and trans-Golgi localization [O] . Kanwarpal S. Dhugga, Suresh C. Tiwari, Peter M. Ray 1997

机译：一种可能与植物细胞壁合成有关的可逆糖基化多肽（RGP1）：纯化基因克隆和反高尔基体定位
7. A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: Purification, gene cloning, and trans-Golgi localization [O] . K. S. Dhugga, S. C. Tiwari, P. M. Ray 1997

机译：可能参与植物细胞壁合成的可逆性糖基化的多肽（RGP1）：纯化，基因克隆和反式高尔基定位

A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: Purification, gene cloning, and trans-Golgi localization

摘要

著录项

相似文献

相关主题

期刊订阅