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The U5 RNA of trypanosomes deviates from the canonical U5 RNA: The Leptomonas collosoma U5 RNA and its coding gene

机译:锥虫的U5 RNA与规范的U5 RNA有所不同:Leptomonas collosoma U5 RNA及其编码基因

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摘要

Fractionation of the abundant small ribo- nucleoproteins (RNPs) of the trypanosomatid Leptomonas collosoma revealed the existence of a group of unidentified small RNPs that were shown to fractionate differently than the well-characterized trans-spliceosomal RNPs. One of these RNAs, an 80-nt RNA, did not possess a trimethylguanosine (TMG) cap structure but did possess a 5' phosphate terminus and an invariant consensus U5 snRNA loop 1. The gene coding for the RNA was cloned, and the coding region showed 55/100 sequence identity to the recently described U5 homologue of Trypanosoma brucei [Dungan, J. D., Watkins, K. P. & Agabian, N. (1996) EMBO J. 15, 4016-4029].
机译:分离锥虫瘤型Leptomonas collosoma丰富的小核糖核蛋白(RNP)揭示了存在一组未鉴定的小RNP,这些小RNP被证明与特征明确的反跨剪接RNP不同。这些RNA之一是80 nt RNA,不具有三甲基鸟苷(TMG)帽结构,但具有5'磷酸末端和不变的共有U5 snRNA环1。克隆了编码该RNA的基因,并编码该区域与最近描述的布鲁氏锥虫的U5同源物显示55/100序列同一性[Dungan,JD,Watkins,KP和Agabian,N。(1996)EMBO J.15,4016-4029]。

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