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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression
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The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression

机译:枯草芽孢杆菌crh基因编码参与碳分解代谢物阻遏的HPr样蛋白

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摘要

Carbon catabolite repression (CCR) of sev- eral Bacillus subtilis catabolic genes is mediated by ATP- dependent phosphorylation of histidine-containing protein (HPr), a phosphocarrier protein of the phosphoenolpyruvate (PEP): sugar phosphotransferase system. In this study, we report the discovery of a new B. subtilis gene encoding a HPr-like protein, Crh (for catabolite repression HPr), com- posed of 85 amino acids. Crh exhibits 45/100 sequence identity with HPr, but the active site His-15 of HPr is replaced with a glutamine in Crh. Crh is therefore not phosphorylated by PEP and enzyme I, but is phosphorylated by ATP and the HPr kinase in the presence of fructose-1,6-bisphosphate.
机译:几个枯草芽孢杆菌分解代谢基因的碳分解代谢阻遏(CCR)由含组氨酸的蛋白质(HPr)(磷酸烯醇丙酮酸(PEP):糖磷酸转移酶系统的磷酸化载体蛋白)的ATP依赖性磷酸化介导。在这项研究中,我们报告发现了一个新的枯草芽孢杆菌基因,该基因编码一个由85个氨基酸组成的HPr样蛋白Crh(用于分解代谢物抑制HPr)。 Crh与HPr表现出45/100序列同一性,但是HPr的活性位点His-15被Crh中的谷氨酰胺替代。因此,Crh不会被PEP和酶I磷酸化,而会在果糖1,6-双磷酸酯的存在下被ATP和HPr激酶磷酸化。

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