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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Analysis of the role of the Spitzenkorper in fungal morphogenesis by computer simulation of apical branching in Aspergillus niger
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Analysis of the role of the Spitzenkorper in fungal morphogenesis by computer simulation of apical branching in Aspergillus niger

机译:通过计算机模拟黑曲霉的顶端分支,分析Spitzenkorper在真菌形态发生中的作用

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摘要

High-resolution video microscopy, image analysis, and computer simulation were used to study the role of the Spitzenkorper (Spk) in apical branching of ramosa-1, a temperature-sensitive mutant of Aspergillus niger. A shift to the restrictive temperature led to a cytoplasmic contraction that destabilized the Spk, causing its disappearance. After a short transition period, new Spk appeared where the two incipient apical branches emerged. Changes in cell shape, growth rate, and Spk position were recorded and transferred to the FUNGUS SIMULATOR program to test the hypothesis that the Spk functions as a vesicle supply center (VSC).
机译:高分辨率视频显微镜,图像分析和计算机模拟用于研究Spitzenkorper(Spk)在黑曲霉对温度敏感的突变体ramosa-1的顶端分支中的作用。限制温度的变化导致细胞质收缩,使Spk不稳定,从而使其消失。在短暂的过渡期后,新的Spk出现了,这是两个最初的根尖分支出现的地方。记录细胞形状,生长速率和Spk位置的变化,并将其转移到FUNGUS SIMULATOR程序中,以检验Spk充当囊泡供应中心(VSC)的假设。

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