mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm

Doris Kraemer; Gunter Blobel

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm

【24h】

mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm

机译：mRNA结合蛋白mrnp 41既位于细胞核又位于细胞质

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We have identified and molecularly charac- terized a human protein with a Mr of 40,880 Da. After UV irradiation of HeLa cells, this protein was cross-linked to poly(A)-containing mRNA and was therefore designated mrnp 41 (for mRNA binding protein of 41 kDa). Cell fractionation and immunoblotting showed mrnp 41 in both the cytoplasm and the nucleus and particularly in the nuclear envelope. Immunofluorescence microscopy localized mrnp 41 to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork- like structures throughout the cytoplasm. The cytoplasmic meshwork staining was disrupted by prior treatment of cells with the actin filament- or microtubule-disrupting drugs cytochalasin or nocodazole, respectively, suggesting associa- tion of mrnp 41 with the cytoskeleton.

机译：我们已经鉴定出了40880 Da先生的人类蛋白质并对其进行了分子表征。在紫外线照射HeLa细胞后，该蛋白质与含聚A的mRNA交联，因此命名为mrnp 41（用于41 kDa的mRNA结合蛋白）。细胞分级分离和免疫印迹显示在细胞质和细胞核中，特别是在核包膜中的mrnp 41。免疫荧光显微镜将mrnp 41定位于核质中的不同病灶，核边缘以及整个细胞质中的网状结构。事先用破坏肌动蛋白的细丝或微管的药物细胞松弛素或诺考达唑分别处理细胞，破坏了细胞质网状染色，表明mrnp 41与细胞骨架有关。

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第17期|p.9119-9124|共6页
作者
Doris Kraemer; Gunter Blobel;
展开▼
作者单位

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词

相似文献

外文文献
中文文献
专利

1. Pre-mRNA Splicing Imprints mRNA in the Nucleus with a Novel RNA-Binding Protein that Persists in the Cytoplasm [J] . Naoyuki Kataoka, Jeongsik Yong, V. Narry Kim, Molecular cell . 2000,第3期

机译：前mRNA剪接与持久存在于细胞质中的新型RNA结合蛋白印记核中的mRNA。
2. Lipid-binding proteins modulate ligand-dependent trans-activation by peroxisome proliferator-activated receptors and localize to the nucleus as well as the cytoplasm. [J] . Helledie T, Antonius M, Sorensen RV, Journal of Lipid Research . 2000,第11期

机译：脂质结合蛋白通过过氧化物酶体增殖物激活的受体调节依赖配体的反式激活，并定位于细胞核以及细胞质。
3. Yeast poly(A)-binding protein Pab1 shuttles between the nucleus and the cytoplasm and functions in mRNA export. [J] . Brune C, Munchel SE, Fischer N, RNA . 2005,第4期

机译：酵母聚（A）结合蛋白Pab1在细胞核和细胞质之间穿梭，并在mRNA输出中发挥作用。
4. Transport of Insulin-like Growth Factor 1 in Intervertebral Disc: Effect of Binding Interactions and Inhomogeneous Distribution of Binding Proteins in Annulus Fibrosus and Nucleus Pulposus [C] . Francesco Travascio, Chun Yuh Huang, Wei Yong Gu ASME summer bioengineering conference;SBC2010 . 2010

机译：胰岛素样生长因子1在椎间盘中的运输：纤维环和髓核中的结合相互作用和结合蛋白的不均匀分布的影响
5. mRNA binding proteins and microRNAs: Dynamic organization of the mRNP infrastructure [D] . Lager, Patrick James 2006

机译：mRNA结合蛋白和microRNA：mRNP基础结构的动态组织
6. mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm [O] . Doris Kraemer, Günter Blobel 1997

机译：mRNA结合蛋白mrnp 41既位于细胞核又位于细胞质
7. mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm [O] . D. Kraemer, G. Blobel 1997

机译：mRNA结合蛋白MRNP 41定位于核和细胞质

mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm

摘要

著录项

相似文献

相关主题

期刊订阅