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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Late memory-related genes in the hippocampus revealed by RNA fingerprinting
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Late memory-related genes in the hippocampus revealed by RNA fingerprinting

机译：RNA指纹显示海马中与记忆相关的晚期基因

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摘要

Although long-term memory is thought to require a cellular program of gene expression and increased protein synthesis, the identity of proteins critical for associa- tive memory is largely unknown. We used RNA fingerprinting to identify candidate memory-related genes (MRGs), which were up-regulated in the hippocampus of water maze-trained rats, a brain area that is critically involved in spatial learning. Two of the original 10 candidate genes implicated by RNA fingerprinting, the rat homolog of the ryanodine receptor type-2 and glutamate dehydrogenase (EC 1.4.1.3), were fur- ther investigated by Northern blot analysis, reverse transcrip- tion-PCR, and in situ hybridization and confirmed as MRGs with distinct temporal and regional expression. Successive RNA screening as illustrated here may help to reveal a spectrum of MRGs as they appear in distinct domains of memory storage.
机译：尽管长期记忆被认为需要基因表达和增加蛋白质合成的细胞程序，但对于关联记忆至关重要的蛋白质的身份却仍然未知。我们使用RNA指纹技术来识别候选记忆相关基因（MRG），这些基因在水迷宫训练的大鼠的海马中上调，该大鼠的大脑区域在空间学习中至关重要。通过RNA指纹图谱牵涉的最初的10个候选基因中的两个，即鼠李糖苷2型受体和谷氨酸脱氢酶的同源物（EC 1.4.1.3），通过Northern印迹分析，逆转录PCR和原位杂交，并确认为具有不同时间和区域表达的MRG。如此处所示，连续的RNA筛选可能有助于揭示一系列MRG，因为它们出现在内存存储的不同域中。

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