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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Barriers to recombination between closely related bacteria: MutS and RecBCD inhibit recombination between Salmonella typhimurium and Salmonella typhi
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Barriers to recombination between closely related bacteria: MutS and RecBCD inhibit recombination between Salmonella typhimurium and Salmonella typhi

机译:紧密相关细菌之间的重组障碍:MutS和RecBCD抑制鼠伤寒沙门氏菌和鼠伤寒沙门氏菌之间的重组

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摘要

Previous studies have shown that inactiva- tion of the MutS or MutL mismatch repair enzymes increases the efficiency of homeologous recombination between Esche- richia coli and Salmonella typhimurium and between S. typhi- murium and Salmonella typhi. However, even in mutants defective for mismatch repair the recombination frequencies are 102- to 103-fold less than observed during homologous recombination between a donor and recipient of the same species. In addition, the length of DNA exchanged during transduction between S. typhimurium and S. typhi is less than in transductions between strains of S. typhimurium.
机译:先前的研究表明,MutS或MutL错配修复酶的失活提高了大肠杆菌和鼠伤寒沙门氏菌之间以及鼠伤寒沙门氏菌和鼠伤寒沙门氏菌之间同源重组的效率。然而,即使在错配修复缺陷的突变体中,重组频率也比​​相同物种的供体和受体之间的同源重组期间的观察到的频率低102-103倍。另外,在鼠伤寒沙门氏菌和鼠伤寒沙门氏菌之间的转导过程中交换的DNA的长度小于鼠伤寒沙门氏菌菌株之间的转导中的DNA的长度。

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