...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >On the reaction mechanism of L-lactate oxidase: Quantitative structure-activity analysis of the reaction with para-substituted-L-mandelates
【24h】

On the reaction mechanism of L-lactate oxidase: Quantitative structure-activity analysis of the reaction with para-substituted-L-mandelates

机译：关于L-乳酸氧化酶的反应机理：对-取代-L-扁桃酸酯反应的定量结构-活性分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The rate constants for reduction of the fla- voenzyme, L-lactate oxidase, and a mutant (in which alanine 95 is replaced by glycine), by a series of para-substituted mandelates, in both the 2-~1H- and 2-~2H- forms, have been measured by rapid reaction spectrophotometry. In all cases, significant isotope effects (~1H/~2H = 3-7) on the rate constants of flavin reduction were found, indicating that flavin reduc- tion is a direct measure of αC-H bond breakage. The rate constants show only a small influence of the electronic characteristics of the substituents, but show a good correla- tion when combined with some substituent volume parame- ters. A surprisingly good correlation is found with the molec- ular mass of the substrate.
机译：黄酮酶，L-乳酸氧化酶和突变体（其中丙氨酸95被甘氨酸替代），一系列的对位取代扁桃酸盐在2-〜1H-和2-H还原的速率常数已通过快速反应分光光度法测量了〜2H-形式。在所有情况下，均发现了对黄素还原速率常数的显着同位素影响（〜1H /〜2H = 3-7），这表明黄素还原是αC-H键断裂的直接量度。速率常数对取代基的电子特性影响很小，但与某些取代基体积参数结合时，则显示出良好的相关性。发现与底物的分子质量有着惊人的良好相关性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号