Phage display of a catalytic antibody to optimize affinity for transition-state analog binding

Manuel Baca; Thomas S. Scanlan; Robert C. Stephenson

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Phage display of a catalytic antibody to optimize affinity for transition-state analog binding

机译：噬菌体展示催化抗体，以优化对过渡态类似物结合的亲和力

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Catalytic antibodies have shown great prom- ise for catalyzing a tremendously diverse set of natural and unnatural chemical transformations. However, few catalytic antibodies have efficiencies that approach those of natural enzymes. In principle, random mutagenesis procedures such as phage display could be used to improve the catalytic activities of existing antibodies; however, these studies have been hampered by difficulties in the recombinant expression of antibodies. Here, we have grafted the antigen binding loops from a murine-derived catalytic antibody, 17E8, onto a human antibody framework in an effort to overcome difficulties associated with recombinant expression and phage display of this antibody.

机译：催化抗体已显示出极大的潜力，可催化多种多样的自然和非自然化学转化。然而，几乎没有催化抗体具有接近天然酶的效率。原则上，可以使用随机诱变程序（例如噬菌体展示）来提高现有抗体的催化活性。然而，这些研究由于抗体的重组表达困难而受到阻碍。在这里，我们已经将鼠源催化抗体17E8的抗原结合环移植到人抗体框架上，以克服与该抗体的重组表达和噬菌体展示相关的困难。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第19期|p.10063-10068|共6页
作者
Manuel Baca; Thomas S. Scanlan; Robert C. Stephenson;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. Selection of phage‐displayed Fab antibodies on the active conformation of Ras yields a high affinity conformation‐specific antibody preventing the binding of c‐Raf kinase to Ras [J] . de Bruiuml, ne Adriaan P., Hoogenboom Hennie R., FEBS Letters . 1999,第1a2期

机译：选择具有Ras活性构象的噬菌体展示Fab抗体可产生高亲和力构象特异性抗体，从而防止c-Raf激酶与Ras结合
2. EF-Tu binding peptides identified, dissected, and affinity optimized by phage display [J] . Murase K., Morrison KL., Tam PY., Chemistry & biology . 2003,第2期

机译：通过噬菌体展示鉴定，解剖和亲和力优化EF-Tu结合肽
3. AFFINITY MATURATION OF A HIGH-AFFINITY HUMAN MONOCLONAL ANTIBODY AGAINST THE THIRD HYPERVARIABLE LOOP OF HUMAN IMMUNODEFICIENCY VIRUS - USE OF PHAGE DISPLAY TO IMPROVE AFFINITY AND BROADEN STRAIN REACTIVITY [J] . Thompson J., Tung JS., Chan C., Journal of Molecular Biology . 1996,第1期

机译：对抗人类免疫缺陷病毒的第三超变循环的高亲和力人类单克隆抗体的亲和力成熟-利用噬菌体展示提高亲和力和血红蛋白应变能力
4. Directed evolution of catalytic antibodies in phage-displayed combinatorial libraries [C] . China-Japan-Korea Joint Symposium on Enzyme Engineering . 2004

机译：噬菌体展示组合文库中催化抗体的定向进化
5. Optimization of protein purification in chromatography processes: Selection of optimum affinity tags from a phage display library and mathematical modeling with immobilized metal affinity chromatography as a case study. [D] . Patwardhan, Anshuman Vidvadhar. 1997

机译：色谱过程中蛋白质纯化的优化：从噬菌体展示库中选择最佳亲和标签，并以固定化金属亲和色谱为例进行数学建模。
6. Phage display of a catalytic antibody to optimize affinity for transition-state analog binding [O] . Manuel Baca, Thomas S. Scanlan, Robert C. Stephenson, 1997

机译：噬菌体展示催化抗体以优化对过渡态类似物结合的亲和力
7. Phage display of a catalytic antibody to optimize affinity for transition-state analog binding [O] . M. Baca, T. S. Scanlan, R. C. Stephenson, 1997

机译：催化抗体的噬菌体展示，优化用于过渡态模拟结合的亲和力
8. Specificity and Binding Affinity of an Anti-Crotoxin Combinatorial AntibodySelected from a Phage-Displayed Library [R] . Meng, J., John, T. R., Kaiser, I. I. 1995

机译：从噬菌体展示文库中选择的抗Crotoxin组合抗体的特异性和结合亲和力

Phage display of a catalytic antibody to optimize affinity for transition-state analog binding

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