B cell receptor-associated protein α4 displays rapamycin-sensitive binding directly to the catalytic subunit of protein phosphatase 2A

Kohei Murata; Jun Wu; David L. Brautigan

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B cell receptor-associated protein α4 displays rapamycin-sensitive binding directly to the catalytic subunit of protein phosphatase 2A

机译：B细胞受体相关蛋白α4直接对蛋白磷酸酶2A的催化亚基显示雷帕霉素敏感结合

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Recently, TAP42 was isolated as a high copy suppressor of sit4~-a yeast phosphatase related to protein phosphatase 2A (PP2A). TAP42 is related to the murine α4 protein, which was discovered independently by its association with Ig-α in the B cell receptor complex. Herein we show that a glutathione S-transferase (GST)-α 4 fusion protein bound the catalytic subunit (C) of human PP2A from monomeric or multimeric preparations of PP2A in a "pull-down" assay. In an overlay assay, the GST-α4 protein bound to the phosphor- ylated and unphosphorylated forms of C that were separated in two-dimensional gels and immobilized on filters. The results show direct and exclusive binding of α4 to C.

机译：最近，分离出TAP42作为sit4-与蛋白质磷酸酶2A（PP2A）有关的酵母磷酸酶的高拷贝抑制剂。 TAP42与鼠类α4蛋白有关，该蛋白是通过与B细胞受体复合物中的Ig-α结合而独立发现的。本文中我们显示了谷胱甘肽S-转移酶（GST）-α4融合蛋白在“下拉”测定法中结合了来自PP2A单体或多聚制剂的人PP2A催化亚基（C）。在覆盖分析中，GST-α4蛋白与C的磷酸化和未磷酸化形式结合，后者在二维凝胶中分离并固定在滤膜上。结果表明α4直接和排他地结合到C。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第20期|p.10624-10629|共6页
作者
Kohei Murata; Jun Wu; David L. Brautigan;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. The Catalytic Subunit of Protein Phosphatase 2A (PP2Ac) Promotes DNA Hypomethylation by Suppressing the Phosphorylated Mitogen-activated Protein Kinase/Extracellular Signal-regulated Kinase (ERK) Kinase (MEK)/Phosphorylated ERK/DNMT1 Protein Pathway in T-cells from Controls and Systemic Lupus Erythematosus Patients [J] . Katsue Sunahori, Kamalpreet Nagpal, Christian Hedrich, The Journal of biological chemistry . 2013,第30期

机译：蛋白质磷酸酶2a（pp2ac）的催化亚基通过抑制来自对照和系统性的T细胞中的磷酸化的丝裂剂活化的蛋白激酶/细胞外信号调节激酶（ERK）激酶（MEK）/ DNMT1蛋白途径来促进DNA甲基化。狼疮红斑病患者
2. Identification of binding sites on the regulatory A subunit of protein phosphatase 2A for the catalytic C subunit and for tumor antigens of simian virus 40 and polyomavirus. [J] . R Ruediger, D Roeckel, J Fait, Molecular and Cellular Biology . 1992,第11期

机译：鉴定蛋白磷酸酶2A的调节性A亚基上的催化C亚基以及猿猴病毒40和多瘤病毒的肿瘤抗原的结合位点。
3. Cross talk between p38MAPK and ERK is mediated through MAPK-mediated protein phosphatase 2A catalytic subunit α and MAPK phosphatase-1 expression in human leukemia U937 cells [J] . Liu W.-H., Chen Y.-J., Cheng T.-L., Cellular Signalling . 2013,第9期

机译：p38MAPK和ERK之间的串扰是通过MAPK介导的蛋白磷酸酶2A催化亚基α和MAPK磷酸酶-1在人白血病U937细胞中的表达而介导的
4. Protein Interaction Partners of Protein Phosphatase 2A Catalytic Subunit in Rat β-Islet cells Using Quantitative Mass Spectrometry [C] . Divyasri Damacharla, Khadija Syeda, Xiangmin Zhang, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：使用定量质谱法在大鼠β-胰岛细胞中蛋白质磷酸酶2A催化亚基的蛋白质相互作用伴侣
5. Isolation and characterization of a dominant-negative protein phosphatase 2A catalytic subunit active site mutant. [D] . Lizotte, Donna L. 2004

机译：显性阴性蛋白磷酸酶2A催化亚基活性位点突变体的分离和表征。
6. B cell receptor-associated protein α4 displays rapamycin-sensitive binding directly to the catalytic subunit of protein phosphatase 2A [O] . Kohei Murata, Jun Wu, David L. Brautigan 1997

机译：B细胞受体相关蛋白α4直接对雷帕霉素敏感的蛋白磷酸酶2A催化亚基结合
7. The Catalytic Subunit of Protein Phosphatase 2A (PP2Ac) Promotes DNA Hypomethylation by Suppressing the Phosphorylated Mitogen-activated Protein Kinase/Extracellular Signal-regulated Kinase (ERK) Kinase (MEK)/Phosphorylated ERK/DNMT1 Protein Pathway in T-cells from Controls and Systemic Lupus Erythematosus Patients [O] . Katsue Sunahori, Kamalpreet Nagpal, Christian M. Hedrich, 2013

机译：蛋白质磷酸酶2a（pp2ac）的催化亚基通过抑制来自对照和系统性的T细胞中的磷酸化的丝裂剂活化的蛋白激酶/细胞外信号调节激酶（ERK）激酶（MEK）/ DNMT1蛋白途径来促进DNA甲基化。狼疮红斑病患者
8. Solution structure analysis of the conformational changes that occur upon the binding of the protein kinase inhibitor peptide to the catalytic subunit of the cAMP dependent protein kinase [R] . Mitchell, R. D. , Walsh, D. A. , Olah, G. A. , 1994

机译：蛋白激酶抑制剂肽与camp依赖性蛋白激酶催化亚基结合后发生的构象变化的溶液结构分析

B cell receptor-associated protein α4 displays rapamycin-sensitive binding directly to the catalytic subunit of protein phosphatase 2A

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