Conformational fluctuations in single DNA molecules

Stefan Wennmalm; Lars Edman; Rudolf Rigler

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Conformational fluctuations in single DNA molecules

机译：单个DNA分子的构象波动

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Measurement of fluorescent lifetimes of dye- tagged DNA molecules reveal the existence of different con- formations. Conformational fluctuations observed by fluo- rescence correlation spectroscopy give rise to a relaxation behavior that is described by "stretched" exponentials and indicates the presence of a distribution of transition rates between two conformations. Whether this is an inhomoge- neous distribution, where each molecule contributes with its own reaction rate to the overall distribution, or a homoge- neous distribution, where the reaction rate of each molecule is time-dependent, is not yet known. We used a tetramethyl- rhodamine-linked 217-bp DNA oligonucleotide as a probe for conformational fluctuations.

机译：染料标记的DNA分子的荧光寿命的测量揭示了不同构象的存在。荧光相关光谱法观察到的构象波动引起松弛行为，该行为由“拉伸”指数描述，表明存在两种构象之间的过渡速率分布。究竟是每个分子以自己的反应速率贡献于总体分布的非均质分布，还是每个分子的反应速率是时间依赖性的均质分布的，这一点尚不清楚。我们使用四甲基-若丹明连接的217-bp DNA寡核苷酸作为构象波动的探针。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第20期|p.10641-10646|共6页
作者
Stefan Wennmalm; Lars Edman; Rudolf Rigler;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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Conformational fluctuations in single DNA molecules

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