Analysis of a peptide hormone-receptor interaction in the yeast two-hybrid system

Jianwei Zhu; C. Ronald Kahn

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Analysis of a peptide hormone-receptor interaction in the yeast two-hybrid system

机译：酵母双杂交系统中肽激素-受体相互作用的分析

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Interaction between a peptide hormone and extracellular domains of its receptor is a crucial step for initiation of hormone action. We have developed a modifica- tion of the yeast two-hybrid system to study this interaction and have used it to characterize the interaction of insulin-like growth factor 1 (IGF-1) with its receptor by using GAL4 transcriptional regulation with a β-galactosidase assay as readout. In this system, IGF-1 and proIGF-1 bound to the cysteine-rich domain, extracellular domain, or entire IGF-1 proreceptor. This interaction was specific.

机译：肽激素与其受体的胞外域之间的相互作用是引发激素作用的关键步骤。我们开发了一种酵母双杂交系统的修饰物，以研究这种相互作用，并通过使用GAL4转录调控和β-受体蛋白来表征胰岛素样生长因子1（IGF-1）与它的受体之间的相互作用。半乳糖苷酶测定法作为读数。在该系统中，IGF-1和proIGF-1与富含半胱氨酸的结构域，细胞外结构域或整个IGF-1受体结合。这种相互作用是特定的。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第24期|p.13063-13068|共6页
作者
Jianwei Zhu; C. Ronald Kahn;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. Protein-peptide interactions analyzed with the yeast two-hybrid system. [J] . Yang M, Wu Z, Fields S Nucleic Acids Research . 1995,第7期

机译：用酵母双杂交系统分析蛋白质-肽的相互作用。
2. Protein-peptide interactions analyzed with the yeast two-hybrid system [J] . Meijia Yang, Stanley Fields, Zining Wu Nucleic acids research . 1995,第7期

机译：用酵母双杂交系统分析蛋白质-肽的相互作用
3. Protein-peptide interactions analyzed with the yeast two-hybrid system [J] . Meijia Yang, Stanley Fields, Zining Wu Nucleic acids research . 1995,第7期

机译：用酵母双杂交系统分析蛋白质-肽的相互作用
4. FUNCTIONAL ANALYSIS OF THE NIFA GAP DOMAIN OF AZOSPIRILLUM BRASILENSE: EFFECT OF TYR-PHE MUTATIONS IN NIFA AND INTERACTION OF THE MUTATED PROTEINS WITH GLNB USING THE YEAST TWO-HYBRID SYSTEM [C] . Sanfeng Chen, Li Liu, Xiaoyu Zhou, International Nitrogen Fixation Congress . 2005

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5. Competitive yeast three-hybrid assays and non-competitive yeast two-hybrid assays to measure relative strength of protein interactions during the gametophytic self-incompatibility response in Petunia hybrida. [D] . Shrestha, Pratima. 2010

机译：竞争性酵母三杂交测定法和非竞争性酵母两杂交测定法，用于测量矮牵牛配子体自交不亲和反应中蛋白质相互作用的相对强度。
6. Analysis of a peptide hormone–receptor interaction in the yeast two-hybrid system [O] . Jianwei Zhu, C. Ronald Kahn 1997

机译：酵母双杂交系统中肽激素-受体相互作用的分析
7. Protein-peptide interactions analyzed with the yeast two-hybrid system. [O] . Yang, M, Wu, Z, Fields, S 1995

机译：用酵母双杂交系统分析蛋白质-肽相互作用。

Analysis of a peptide hormone-receptor interaction in the yeast two-hybrid system

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