Direct repeat sequences in the Streptomyces chitinase-63 promoter direct both glucose repression and chitin induction

Xiangyang Ni; Janet Westpheling

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Direct repeat sequences in the Streptomyces chitinase-63 promoter direct both glucose repression and chitin induction

机译：链霉菌几丁质酶-63启动子中的直接重复序列既指导葡萄糖抑制又指导几丁质诱导

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The chi63 promoter directs glucose-sensitive, chitin-dependent transcription of a gene involved in the utilization of chitin as carbon source. Analysis of 5 and 3 deletions of the promoter region revealed that a 350-bp segment is sufficient for wild-type levels of expression and regulation. The analysis of single base changes throughout the promoter region, introduced by random and site-directed mutagenesis, identified several sequences to be important for activity and regulation. Single base changes at -10, -12, -32, -33, -35, and -37 upstream of the transcription start site resulted in loss of activity from the promoter, suggesting that bases in these positions are important for RNA polymerase interaction.

机译：chi63启动子指导涉及对几丁质作为碳源利用的基因的葡萄糖敏感，几丁质依赖性转录。对启动子区域的5和3个缺失的分析表明，一个350 bp的片段足以满足野生型表达和调控水平。由随机和定点诱变引入的整个启动子区域的单碱基变化分析确定了几个对活性和调控很重要的序列。转录起始位点上游的-10，-12，-32，-33，-35和-37处的单碱基变化导致启动子活性丧失，表明这些位置的碱基对于RNA聚合酶相互作用很重要。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第24期|p.13116-13121|共6页
作者
Xiangyang Ni; Janet Westpheling;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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机译：链霉菌sp EC3的木聚糖酶C启动子区域中保守的反向重复序列的定点诱变
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5. Analyses of cis- and trans-acting elements involved in glucose repression and transcriptional regulation of chitin utilization in Streptomyces lividans. [D] . Ni, Xiangyang. 1997

机译：分析在青霉链霉菌中参与葡萄糖阻抑和几丁质利用的转录调控的顺式和反式作用元件。
6. Direct repeat sequences in the Streptomyces chitinase-63 promoter direct both glucose repression and chitin induction [O] . Xiangyang Ni, Janet Westpheling 1997

机译：链霉菌几丁质酶-63启动子中的直接重复序列指导葡萄糖阻抑和几丁质诱导
7. Site-directed mutagenesis of conserved inverted repeat sequences in the xylanase C promoter region from Streptomyces sp EC3 [O] . Giannotta, F., Georis, J., Rigali, Sébastien, 2003

机译：链霉菌sp EC3的木聚糖酶C启动子区域中保守的反向重复序列的定点诱变

Direct repeat sequences in the Streptomyces chitinase-63 promoter direct both glucose repression and chitin induction

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