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Genetic recombination of poliovirus in a cell-free system

机译:脊髓灰质炎病毒在无细胞系统中的遗传重组

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摘要

Genetic recombination of plus-strand RNA viruses is an important process for promoting genetic varia- tion. By using genetically marked poliovirus RNAs, we have demonstrated that genetic recombination can occur in a cell-free system that generates infective virus from added poliovirus RNA. Recombinant polioviruses were isolated, and the region of crossing over was roughly mapped. Recombi- nants could be isolated even under conditions where the yield of viruses from one of the parental RNAs was depressed to levels comparable to or less than the yield of recombinant viruses, an observation suggesting that only one of the recom- bining RNAs needs to be replication-competent.
机译:正链RNA病毒的遗传重组是促进遗传变异的重要过程。通过使用遗传标记的脊髓灰质炎病毒,我们已经证明遗传重组可以发生在无细胞的系统中,该系统从添加的脊髓灰质炎病毒RNA产生感染性病毒。分离出重组脊髓灰质炎病毒,并粗略划定交叉区域。即使将亲本RNA之一的病毒产量降低到与重组病毒的产量相当或更低的水平,也可以分离出重组子,这一观察表明,仅需要重组RNA之一即可。具有复制能力。

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