Escherichia coli RNA polymerase terminates transcription efficiently at rho-independent terminators on single-stranded DNA templates

Susan M. Uptain; Michael J. Chamberlin

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Escherichia coli RNA polymerase terminates transcription efficiently at rho-independent terminators on single-stranded DNA templates

机译：大肠杆菌RNA聚合酶可有效终止单链DNA模板上不依赖于rho的终止子的转录

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Several models have been proposed for the mechanism of transcript termination by Escherichia coli RNA polymerase at rho-independent terminators. Yager and von Hippel (Yager, T. D. & von Hippel, P. H. (1991) Biochemistry 30, 1097-118) postulated that the transcription complex is stabilized by enzyme-nucleic acid interactions and the favor- able free energy of a 12-bp RNA-DNA hybrid but is destabi- lized by the free energy required to maintain an extended transcription bubble. Termination, by their model, is viewed simply as displacement of the RNA transcript from the hybrid helix by reformation of the DNA helix.

机译：已经提出了几种用于在不依赖rho的终止子上通过大肠杆菌RNA聚合酶终止转录的机制的模型。 Yager和von Hippel（Yager，TD＆von Hippel，PH（1991）Biochemistry 30，1097-118）假设转录复合物通过酶-核酸相互作用和12 bp RNA- DNA杂种，但由于维持延长的转录气泡所需的自由能而不稳定。根据他们的模型，终止被简单地看作是通过重组DNA螺旋将RNA转录物从杂种螺旋中置换出来。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第25期|p.13548-13553|共6页
作者
Susan M. Uptain; Michael J. Chamberlin;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. Processing of the Escherichia coli leuX tRNA transcript, encoding tRNALeu5, requires either the 3′→5′ exoribonuclease polynucleotide phosphorylase or RNase P to remove the Rho-independent transcription terminator [J] . Bijoy K. Mohanty, Sidney R. Kushner Nucleic acids research . 2010,第2期

机译：编码tRNALeu5的大肠杆菌leuX tRNA转录产物的加工需要3'→5'外核糖核酸酶多核苷酸磷酸化酶或RNase P来去除非Rho依赖性转录终止子
2. Processing of the Escherichia coli leuX tRNA transcript, encoding tRNALeu5, requires either the 3′→5′ exoribonuclease polynucleotide phosphorylase or RNase P to remove the Rho-independent transcription terminator [J] . Bijoy K. Mohanty, Sidney R. Kushner Nucleic acids research . 2010,第2期

机译：编码tRNALeu5的大肠杆菌leuX tRNA转录产物的加工需要3'→5'外核糖核酸酶多核苷酸磷酸化酶或RNase P来去除非Rho依赖性转录终止子
3. RNase E-based degradosome modulates polyadenylation of mRNAs after Rho-independent transcription terminators in Escherichia coli [J] . Mildenhall Kristen B., Wiese Nicholas, Chung Daewhan, Molecular Microbiology . 2016,第4期

机译：基于Rnase E的降解体在大肠杆菌中不依赖Rho的转录终止子后调节mRNA的聚腺苷酸化
4. Cell-free incorporation of photo-crosslinkable p-benzoyl-L-phenylalanine into the polymerase subunits α and ε of Escherichia coli DNA polymerase III [C] . Kiyoshi Ozawa, Gottfried Otting, Nicholas E. Dixon Incorporating of the Australian Society for Biochemistry and Molecular Biology Combio . 2009

机译：无细胞掺入光交联的p-苯甲酰基-1-苯丙氨酸进入聚合酶亚基α和ε的大肠杆菌DNA聚合酶III
5. Key conformational changes of Escherichia coli RNA polymerase and promoter DNA in transcription initiation. [D] . Drennan, Amanda Christine. 2012

机译：大肠杆菌RNA聚合酶和启动子DNA在转录起始过程中的关键构象变化。
6. Escherichia coli RNA polymerase terminates transcription efficiently at rho-independent terminators on single-stranded DNA templates [O] . Susan M. Uptain, Michael J. Chamberlin 1997

机译：大肠杆菌RNA聚合酶可有效终止单链DNA模板上不依赖于rho的终止子的转录
7. Escherichia coli RNA polymerase terminates transcription efficiently at rho-independent terminators on single-stranded DNA templates [O] . S. M. Uptain, M. J. Chamberlin 1997

机译：大肠杆菌RNA聚合酶在单链DNA模板上有效地终止于独立于rho的终止子转录

Escherichia coli RNA polymerase terminates transcription efficiently at rho-independent terminators on single-stranded DNA templates

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