In vivo examination of membrane protein localization and degradation with green fluorescent protein

Randolph Y. Hampton; Ann Koning; Robin Wright

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In vivo examination of membrane protein localization and degradation with green fluorescent protein

机译：膜蛋白定位和绿色荧光蛋白降解的体内检查

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To test the utility of green fluorescent pro- tein (GFP) as an in vivo reporter protein when fused to a membrane domain, we made a fusion protein between yeast hydroxymethylglutaryl-CoA reductase and GFP. Fusion pro- teins displayed spatial localization and regulated degradation consistent with the native hydroxymethylglutaryl-CoA reduc- tase proteins. Thus, GFP should be useful in the study of both membrane protein localization and protein degradation in vivo.

机译：为了测试绿色荧光蛋白（GFP）与膜结构域融合时作为体内报道蛋白的效用，我们在酵母羟甲基戊二酰辅酶A还原酶和GFP之间制备了融合蛋白。融合蛋白显示出与天然羟甲基戊二酰辅酶A还原酶蛋白一致的空间定位和受控降解。因此，GFP在体内膜蛋白定位和蛋白降解研究中均应有用。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第2期|p.828-833|共6页
作者
Randolph Y. Hampton; Ann Koning; Robin Wright;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Flavin Mononucleotide-Based Fluorescent Reporter Proteins Outperform Green Fluorescent Protein-Like Proteins as Quantitative In Vivo Real-Time Reporters [J] . Thomas Drepper, Robert Huber, Achim Heck, Applied and Environmental Microbiology . 2010,第17期

机译：基于黄素单核苷酸的荧光报道蛋白在体内定量实时报道中的表现优于绿色荧光蛋白样蛋白
2. Flavin Mononucleotide-Based Fluorescent Reporter Proteins Outperform Green Fluorescent Protein-Like Proteins as Quantitative In Vivo Real-Time Reporters [J] . Thomas Drepper, Robert Huber, Achim Heck, Applied and Environmental Microbiology . 2010,第17期

机译：基于黄素单核苷酸的荧光报道蛋白在体内定量实时报道中的表现优于绿色荧光蛋白样蛋白
3. Flavin Mononucleotide-Based Fluorescent Reporter Proteins Outperform Green Fluorescent Protein-Like Proteins as Quantitative In Vivo Real-Time Reporters [J] . Thomas Drepper, Robert Huber, Achim Heck, Applied Microbiology . 2010,第17期

机译：基于黄素单核苷酸的荧光报道蛋白在体内定量实时报道中的表现优于绿色荧光蛋白样蛋白
4. Localization of protein-protein interactions among three fluorescent proteins in a single living cell: Three-color FRET Microscopy [C] . Yuansheng Sun, Cynthia F. Booker, Richard N. Day, Multiphoton microscopy in the biomedical sciences IX . 2009

机译：单个活细胞中三种荧光蛋白之间蛋白质-蛋白质相互作用的定位：三色FRET显微镜
5. Green fluorescent protein in Saccharomyces cerevisiae: Real-time studies of the GAL1 promoter, fusion protein expression and localization/secretion. [D] . Li, Jincai. 2001

机译：酿酒酵母中的绿色荧光蛋白：GAL1启动子，融合蛋白表达和定位/分泌的实时研究。
6. In vivo examination of membrane protein localization and degradation with green fluorescent protein. [O] . R Y Hampton, A Koning, R Wright, 1996

机译：体内检查膜蛋白的定位和绿色荧光蛋白的降解。
7. In vivo examination of membrane protein localization and degradation with green fluorescent protein. [O] . Hampton, R Y, Koning, A, Wright, R, 1996

机译：体内检查膜蛋白的定位和绿色荧光蛋白的降解。

In vivo examination of membrane protein localization and degradation with green fluorescent protein

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