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Comparative analysis of ribonuclease P RNA using gene sequences from natural microbial populations reveals tertiary structural elements

机译:使用天然微生物种群的基因序列对核糖核酸酶P RNA进行比较分析,发现三级结构元素

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摘要

PCR amplification of template DNAs ex- tracted from mixed, naturally occurring microbial popula- tions, using oligonucleotide primers complementary to highly conserved sequences, was used to obtain a large collection of diverse RNase P RNA-encoding genes. An alignment of these sequences was used in a comparative analysis of RNase P RNA secondary and tertiary structure.
机译:通过使用与高度保守的序列互补的寡核苷酸引物,对从混合的,天然存在的微生物种群中提取的模板DNA进行PCR扩增,从而获得了大量的各种RNase P RNA编码基因。这些序列的比对用于RNA酶P RNA二级和三级结构的比较分析。

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