Thiocalsin: A thioredoxin-linked, substrate-specific protease dependent on calcium

Isabelle Besse; Joshua H. Wong; Karoly Kobrehel

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Thiocalsin: A thioredoxin-linked, substrate-specific protease dependent on calcium

机译：硫钙素：一种硫氧还蛋白连接的底物特异性蛋白酶，取决于钙

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We describe a protease, named "thiocalsin," that is activated by calcium but only after reductive activation by thioredoxin, a small protein with a redox-active disulfide group that functions widely in regulation. Thiocalsin ap- peared to be a 14-kDa serine protease that functions inde- pendently of calmodulin. The enzyme, purified from germi- nating wheat grain, specifically cleaved the major indigenous storage proteins, gliadins and glutenins, after they too had been reduced, preferentially by thioredoxin.

机译：我们描述了一种被称为“ thiocalsin”的蛋白酶，其被钙激活，但仅在被硫氧还蛋白还原激活后才被激活，硫氧还蛋白是一种具有氧化还原活性二硫基团的小蛋白，在调节中具有广泛的功能。硫代钙素似乎是一种14kDa的丝氨酸蛋白酶，其功能与钙调蛋白无关。该酶是从发芽的小麦籽粒中纯化的，特别是主要被硫氧还蛋白还原后，会裂解主要的本地贮藏蛋白，麦醇溶蛋白和谷蛋白。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第8期|p.3169-3175|共7页
作者
Isabelle Besse; Joshua H. Wong; Karoly Kobrehel;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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专利

1. A single calcium binding site is crucial for the calcium-dependent thermal stability of thermolysin-like proteases [J] . Veltman OR., Berendsen HJC., Van den Burg B., Biochemistry . 1998,第15期

机译：单个钙结合位点对于类嗜热菌蛋白酶的钙依赖性热稳定性至关重要
2. Gene structure of calcium‐dependent protease retains the ancestral organization of the calcium‐binding protein gene [J] . Emori Yasufumi, Ohno Shigeo, Suzuki Koichi, FEBS Letters . 1986,第2期

机译：钙依赖性蛋白酶的基因结构保留了钙结合蛋白基因的祖先组织
3. Gene structure of calcium‐dependent protease retains the ancestral organization of the calcium‐binding protein gene [J] . Emori Yasufumi, Ohno Shigeo, Suzuki Koichi, FEBS Letters . 1986,第2期

机译：钙依赖性蛋白酶的基因结构保留了钙结合蛋白基因的祖先组织
4. Alkaline Protease Immobilization in Alginate-Calcium Chloride Core-shell Microcapsules [C] . Yuan Jiang, Ming-Di Zhang, Mei-Shuo Zhang, Annual International Workshop on Materials Science and Engineering . 2017

机译：碱性蛋白酶固定在海藻酸钙 - 氯化钙核心壳微胶囊中
5. C-di-GMP Mediated Regulation of the LapA Biofilm Adhesin through Proteolysis by the Calcium-Dependent Cysteine Protease LapG. [D] . Boyd, Chelsea D. 2013

机译：C-di-GMP介导的钙依赖性半胱氨酸蛋白酶LapG通过蛋白水解作用调节LapA生物膜粘附素。
6. Thiocalsin: a thioredoxin-linked substrate-specific protease dependent on calcium. [O] . I Besse, J H Wong, K Kobrehel, 1996

机译：硫钙素：一种硫氧还蛋白连接的依赖于钙的底物特异性蛋白酶。
7. Thiocalsin: a thioredoxin-linked, substrate-specific protease dependent on calcium. [O] . Besse, I, Wong, J H, Kobrehel, K, 1996

机译：硫钙素：一种硫氧还蛋白连接的，依赖于钙的底物特异性蛋白酶。
8. Effects of Intracellular Calcium Chelation on Voltage-Dependent and Calcium-Dependent Currents in Cat Neocortical Neurons [R] . Schwindt, P. C., Spain, W. J., Crill, W. E. 1992

机译：细胞内钙螯合作用对猫新皮层神经元电压依赖性和钙依赖性电流的影响

Thiocalsin: A thioredoxin-linked, substrate-specific protease dependent on calcium

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