Detection and localization of individual antibody-antigen recognition events by atomic force microscopy

Peter Hinterdorfer; Werner Baumgartner; Hermann J. Gruber

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Detection and localization of individual antibody-antigen recognition events by atomic force microscopy

机译：通过原子力显微镜检测和定位单个抗体-抗原识别事件

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摘要

A methodology has been developed for the study of molecular recognition at the level of single events and for the localization of sites on biosurfaces, in combining force microscopy with molecular recognition by specific ligands. For this goal, a sensor was designed by covalently linking an antibody (anti-human serum albumin, polyclonal) via a flex- ible spacer to the tip of a force microscope.

机译：通过将力显微镜与特定配体的分子识别相结合，已开发出一种方法用于研究单事件水平的分子识别以及生物表面位点的定位。为了实现这一目标，设计了一种传感器，方法是通过柔性垫片将抗体（抗人血清白蛋白，多克隆抗体）共价连接到力显微镜的尖端。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第8期|p.3477-3481|共5页
作者
Peter Hinterdorfer; Werner Baumgartner; Hermann J. Gruber;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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专利

1. Detection and localization of single molecular recognition events using atomic force microscopy [J] . Hinterdorfer P, Dufrene YF Nature methods . 2006,第5期

机译：使用原子力显微镜检测和定位单个分子识别事件
2. Detection of magnetic-labeled antibody specific recognition events by combined atomic force and magnetic force microscopy [J] . Xia Hong, Yanmei Liu, Jun Li, Journal of magnetism and magnetic materials . 2009,第17期

机译：通过原子力和磁力显微镜联合检测磁性标记的抗体特异性识别事件
3. In situ single-molecule detection of antibody-antigen binding by tapping-mode atomic force microscopy [J] . Li LY., Chen SF., Oh SJ., Analytical chemistry . 2002,第23期

机译：敲击模式原子力显微镜原位单分子检测抗体-抗原结合
4. MONITORING LOCALIZED THIN-FILM CORROSION EVENTS VIA INTEGRATED OPTICAL AND ATOMIC FORCE MICROSCOPIES [C] . Keith J. Stevenson, Joseph T. Hupp, Susan G. Yan International Symposium on Localized In-Situ Methods for Investigating Electrochemical Interfaces, Oct 20-21, 1999, Honolulu, Hawaii . 1999

机译：通过集成的光学和原子力显微镜监测局部的薄膜腐蚀事件
5. Factors and forces involved in the initial events of bacterial adhesion as monitored by atomic force microscopy. [D] . Razatos, Anneta Panagis. 1999

机译：通过原子力显微镜监测，参与细菌粘附初始事件的因素和作用力。
6. Detection and localization of individual antibody-antigen recognition events by atomic force microscopy. [O] . P Hinterdorfer, W Baumgartner, H J Gruber, 1996

机译：通过原子力显微镜对单个抗体-抗原识别事件的检测和定位。
7. Detection and localization of individual antibody-antigen recognition events by atomic force microscopy. [O] . Hinterdorfer, P, Baumgartner, W, Gruber, H J, 1996

机译：通过原子力显微镜对单个抗体-抗原识别事件的检测和定位。
8. Observation of Localized Corrosion of Ni-Based Alloys Using Coupled Orientation Imaging Microscopy and Atomic Force Microscopy. [R] . Bedrossian, P. J., Schwartz, A. J., Kumar, M., 1999

机译：用耦合取向显微镜和原子力显微镜观察镍基合金的局部腐蚀。

Detection and localization of individual antibody-antigen recognition events by atomic force microscopy

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