The replication initiator protein π of the plasmid R6K specifically interacts with the host-encoded helicase DnaB

Pillarisetty V. A. L. Ratnakar; Bidyut K. Mohanty; Marc Lobert

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The replication initiator protein π of the plasmid R6K specifically interacts with the host-encoded helicase DnaB

机译：质粒R6K的复制起始蛋白π与宿主编码的解旋酶DnaB特异性相互作用

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The replication initiator protein π of plas- mid R6K is known to interact with the seven iterons of the γ origin/enhancer and activate distant replication origins α and β(oriα and oriβ) by π-mediated DNA looping. Here we show that π protein specifically interacts in vitro with the host-encoded helicase DnaB. The site of interaction of π on DnaB has been localized to a 37-aa-long region located between amino acids 151 and 189 of DnaB.

机译：已知质粒R6K的复制起始蛋白π与γ起源/增强子的七个Iteron相互作用，并通过π介导的DNA环化激活远距离复制起点α和β（oriα和oriβ）。在这里，我们显示了π蛋白在体外与宿主编码解旋酶DnaB特异性相互作用。 DnaB上π的相互作用位点已被定位到位于DnaB氨基酸151和189之间的37-aa长区域。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第11期|p.5522-5526|共5页
作者
Pillarisetty V. A. L. Ratnakar; Bidyut K. Mohanty; Marc Lobert;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. Crystal structure of π initiator protein-iteron complex of plasmid R6K: Implications for initiation of plasmid DNA replication [J] . Michael K. Swan, Deepak Bastia, Christopher Davies Proceedings of the National Academy of Sciences of the United States of America . 2006,第49期

机译：质粒R6K的π起始蛋白-铁蛋白复合物的晶体结构：质粒DNA复制起始的意义
2. Binding of DnaA protein to a replication enhancer counteracts the inhibition of plasmid R6K gamma origin replication mediated by elevated levels of R6K pi protein. [J] . F Wu, I Levchenko, M Filutowicz Journal of bacteriology . 1994,第22期

机译：DnaA蛋白与复制增强子的结合抵消了由R6K pi蛋白水平升高介导的质粒R6Kγ起源复制的抑制。
3. Investigations of π Initiator Protein-mediated Interaction between Replication Origins α and γ of the Plasmid R6K [J] . Mukesh Saxena, Samarendra Singh, Shamsu Zzaman, The Journal of biological chemistry . 2010,第8期

机译：π引发剂蛋白介导的复制之间α和γ的质粒R6K的α和γ的研究
4. Unwinding the Structure of the Helicase Prp43 upon Ntr1 Binding by Oxidative Footprinting, Protein-Protein and Protein-RNA Cross-linking and Mass Spectrometry [C] . Romina Hofele, Henning Christian, Ralf Ficner, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：通过氧化足迹，蛋白质 - 蛋白质和蛋白质-RNA交联和质谱法在NTR1结合时展开螺旋酶PRP43的结构
5. Pheromone-interacting replication protein controls enterococcal conjugative plasmid host range and stability through disulfide bonds. [D] . Utter, Bryan. 2012

机译：信息素相互作用复制蛋白通过二硫键控制肠球菌结合质粒的宿主范围和稳定性。
6. The replication initiator protein pi of the plasmid R6K specifically interacts with the host-encoded helicase DnaB. [O] . P V Ratnakar, B K Mohanty, M Lobert, 1996

机译：质粒R6K的复制起始蛋白pi与宿主编码的解旋酶DnaB特异性相互作用。
7. The replication initiator protein pi of the plasmid R6K specifically interacts with the host-encoded helicase DnaB. [O] . Ratnakar, P V, Mohanty, B K, Lobert, M, 1996

机译：质粒R6K的复制起始蛋白pi与宿主编码的解旋酶DnaB特异性相互作用。

The replication initiator protein π of the plasmid R6K specifically interacts with the host-encoded helicase DnaB

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