Subcellular imaging of intramitochondrial Ca~2+ with recombinant targeted aequorin: Significance for the regulation of pyruvate dehydrogenase activity

Guy A. Rutter; Paul Burnett; Rosario Rizzuto

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Subcellular imaging of intramitochondrial Ca~2+ with recombinant targeted aequorin: Significance for the regulation of pyruvate dehydrogenase activity

机译：重组靶向水母发光蛋白对线粒体内Ca〜2 +的亚细胞成像：丙酮酸脱氢酶活性调节的意义

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Specific targeting of the recombinant, Ca~2+- sensitive photoprotein, aequorin to intracellular organelles has provided new insights into the mechanisms of intracel- lular Ca~2+ homeostasis. When applied to small mammalian cells, a major limitation of this technique has been the need to average the signal over a large number of cells. This prevents the identification of inter- or intracellular heteroge- neities.

机译：重组的Ca〜2 +敏感光蛋白水母发光蛋白对细胞内细胞器的特异性靶向，为了解细胞内Ca〜2 +稳态的机制提供了新的见识。当应用于小型哺乳动物细胞时，该技术的主要局限性在于需要对大量细胞进行平均信号处理。这阻止了细胞间或细胞内异质性的鉴定。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第11期|p.5489-5494|共6页
作者
Guy A. Rutter; Paul Burnett; Rosario Rizzuto;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. Role of Ca2+ ions in the regulation of intramitochondrial metabolism in rat heart. Evidence from studies with isolated mitochondria that adrenaline activates the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes by increasing the intramitochondrial concentration of Ca2+ [J] . J G McCormack, R M Denton The biochemical journal . 1984,第1期

机译：Ca2 +离子在调节大鼠心脏线粒体内代谢中的作用。分离线粒体的研究证据表明，肾上腺素可通过增加线粒体内Ca2 +的浓度来激活丙酮酸脱氢酶和2-氧戊二酸脱氢酶复合物。
2. Role of calcium ions in the regulation of intramitochondrial metabolism. Effects of Na+, Mg2+ and ruthenium red on the Ca2+-stimulated oxidation of oxoglutarate and on pyruvate dehydrogenase activity in intact rat heart mitochondria [J] . J G McCormack, N J Edgell, R M Denton The biochemical journal . 1980,第1期

机译：钙离子在调节线粒体内代谢中的作用。 Na +，Mg2 +和钌红对完整大鼠心脏线粒体中Ca2 +刺激的戊二酸氧化和丙酮酸脱氢酶活性的影响
3. Role of Ca2+ ions in the regulation of intramitochondrial metabolism in rat epididymal adipose tissue. Evidence against a role for Ca2+ in the activation of pyruvate dehydrogenase by insulin [J] . J G McCormack, R M Denton, S E Marshall The biochemical journal . 1984,第1期

机译：Ca2 +离子在大鼠附睾脂肪组织线粒体内代谢调控中的作用。关于Ca2 +在胰岛素激活丙酮酸脱氢酶中作用的证据
4. Regulation of the activity of the pyruvate dehydrogenase complex [C] . Robert A. Harris, Melissa M. Bowker-Kinley, Boli Huang, International Symposium on Regulation of Enzyme Activity and Synthesis in Normal and Neoplastic Tissues . 2003

机译：调节丙酮酸脱氢酶复合物的活性
5. Effects of tamoxifen on mitochondrial nitric oxide synthase activity: Alteration in the intramitochondrial calcium(2+) homeostasis. [D] . Joshi, Sandeep S. 2005

机译：他莫昔芬对线粒体一氧化氮合酶活性的影响：线粒体内钙（2+）稳态的改变。
6. Subcellular imaging of intramitochondrial Ca2+ with recombinant targeted aequorin: significance for the regulation of pyruvate dehydrogenase activity. [O] . G A Rutter, P Burnett, R Rizzuto, 1996

机译：重组靶向水母发光蛋白的线粒体内Ca2 +的亚细胞成像：丙酮酸脱氢酶活性调节的意义。
7. Subcellular imaging of intramitochondrial Ca2+ with recombinant targeted aequorin: significance for the regulation of pyruvate dehydrogenase activity. [O] . Rutter, G A, Burnett, P, Rizzuto, R, 1996

机译：重组靶向水母发光蛋白的线粒体内Ca2 +的亚细胞成像：丙酮酸脱氢酶活性调节的意义。
8. Dietary Regulation of Glycolytic Enzymes. Iii. Adaptive Changes in Rat Jejunal Pyruvate Kinase, Phosphofructokinase, Fructosediphosphatase and Glycerol-B3-Phosphate Dehydrogenase [R] . Stifel, F. B., Herman, R. H., Rosenweig, N. S. 1969

机译：膳食调节糖酵解酶。三。大鼠空肠丙酮酸激酶，磷酸果糖激酶，果糖磷酸酶和甘油-B3-磷酸脱氢酶的适应性变化

Subcellular imaging of intramitochondrial Ca~2+ with recombinant targeted aequorin: Significance for the regulation of pyruvate dehydrogenase activity

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