Generation of mice with a 200-kb amyloid precursor protein gene deletion by Cre recombinase-mediated site-specific recombination in embryonic stem cells

Zhi-Wei Li; Gerlinde Stark; Jurgen Gotz

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Generation of mice with a 200-kb amyloid precursor protein gene deletion by Cre recombinase-mediated site-specific recombination in embryonic stem cells

机译：Cre重组酶介导的胚胎干细胞中位点特异性重组产生具有200kb淀粉样前体蛋白基因缺失的小鼠

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Gene disruptions and deletions of up to 20 kb have been generated by homologous recombination with ap- propriate targeting vectors in murine embryonic stem (ES) cells. Because we could not obtain a deletion of about 200 kb in the mouse amyloid precursor protein gene by the classical technique, we employed strategies involving the insertion of loxP sites upstream and downstream of the region to be deleted by homologous recombination and elicited excision of the loxP-flanked region by introduction of a Cre expression vector into the ES cells.

机译：通过与鼠胚胎干（ES）细胞中适当的靶向载体同源重组，产生了高达20 kb的基因破坏和缺失。由于我们无法通过经典技术在小鼠淀粉样蛋白前体蛋白基因中获得约200 kb的缺失，因此我们采用了以下策略：通过同源重组将loxP位点插入待缺失区域的上游和下游，并引起loxP切除通过将Cre表达载体导入ES细胞来形成侧翼区域。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第12期|p.6158-6162|共5页
作者
Zhi-Wei Li; Gerlinde Stark; Jurgen Gotz;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Introduction of Hereditary Disease-Associated Mutations into the β-Amyloid Precursor Protein Gene of Mouse Embryonic Stem Cells: a Comparison of Homologous Recombination Methods [J] . Martin Gschwind, Gerda Huber Molecular and Cellular Biology . 1998,第8期

机译：遗传性疾病相关突变引入小鼠胚胎干细胞的β-淀粉样前体蛋白基因：同源重组方法的比较。
2. Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions [J] . Gagneten S, Le Y, Miller J, Nucleic Acids Research . 1997,第16期

机译：GFP cre融合基因在胚胎干细胞中的简短表达可快速检索位点特异性基因组缺失
3. Effects of huperzine A on amyloid precursor protein processing and beta-amyloid generation in human embryonic kidney 293 APP Swedish mutant cells. [J] . Peng Y, Jiang L, Lee DY, Journal of Neuroscience Research . 2006,第4期

机译：石杉碱甲对人胚肾293 APP瑞典突变细胞中淀粉样前体蛋白加工和β-淀粉样蛋白生成的影响。
4. Proteomic Imaging of amyloids in Brains from Amyloid Precursor Protein (APP) Transgenic Mice in comparison with Human Alzheimer Amyloid [C] . Masaya Ikegawa, Tomohiro Miyasaka, Noriyuki Iwasaki, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：淀粉样蛋白前体蛋白（APP）转基团的淀粉样蛋白淀粉样蛋白蛋白质组学成像与人阿尔茨海默蛋白相比
5. Intracellular trafficking of the Alzheimer beta-amyloid precursor protein regulates beta-amyloid peptide generation. [D] . Greenfield, Jeffrey Peter. 1999

机译：Alzheimerβ-淀粉样蛋白前体蛋白的细胞内运输调节β-淀粉样肽的生成。
6. Generation of mice with a 200-kb amyloid precursor protein gene deletion by Cre recombinase-mediated site-specific recombination in embryonic stem cells. [O] . Z W Li, G Stark, J Götz, 1996

机译：通过Cre重组酶介导的胚胎干细胞中的位点特异性重组产生具有200 kb淀粉样蛋白前体蛋白基因缺失的小鼠。
7. Generation of mice with a 200-kb amyloid precursor protein gene deletion by Cre recombinase-mediated site-specific recombination in embryonic stem cells. [O] . Li, Z W, Stark, G, Götz, J, 1996

机译：通过Cre重组酶介导的胚胎干细胞中的位点特异性重组，产生具有200 kb淀粉样蛋白前体蛋白基因缺失的小鼠。

Generation of mice with a 200-kb amyloid precursor protein gene deletion by Cre recombinase-mediated site-specific recombination in embryonic stem cells

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