Probing the interaction between two single molecules: Fluorescence resonance energy transfer between a single donor and a single acceptor

T. Ha; Th. Enderle; D. F. Ogletree

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Probing the interaction between two single molecules: Fluorescence resonance energy transfer between a single donor and a single acceptor

机译：探索两个单分子之间的相互作用：单个供体和单个受体之间的荧光共振能量转移

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We extend the sensitivity of fluorescence resonance energy transfer (FRET) to the single molecule level by measuring energy transfer between a single donor fluoro- phore and a single acceptor fluorophore. Near-field scanning optical microscopy (NSOM) is used to obtain simultaneous dual color images and emission spectra from donor and acceptor fluorophores linked by a short DNA molecule. Pho- todestruction dynamics of the donor or acceptor are used to determine the presence and efficiency of energy transfer.

机译：通过测量单个供体荧光团和单个受体荧光团之间的能量转移，我们将荧光共振能量转移（FRET）的灵敏度扩展到单个分子水平。近场扫描光学显微镜（NSOM）用于从由短DNA分子连接的供体和受体荧光团同时获得双色图像和发射光谱。供体或受体的光解动力学用于确定能量转移的存在和效率。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第13期|p.6264-6268|共5页
作者
T. Ha; Th. Enderle; D. F. Ogletree;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data [J] . Anja Dietrich, Volker Buschmann, Christian Muller, Reviews in Molecular Biotechnology . 2002,第3期

机译：供体-受体取代的DNA链中的荧光共振能量转移（FRET）和竞争过程：集成和单分子数据的比较研究
2. Multiplexed Interfacial Transduction of Nucleic Acid Hybridization Using a Single Color of Immobilized Quantum Dot Donor and Two Acceptors in Fluorescence Resonance Energy Transfer [J] . W. Russ Algar and Ulrich J. Krull Analytical Chemistry . 2010,第1期

机译：使用单色固定化量子点供体和两个受体在荧光共振能量转移中的核酸杂交的多路界面转导
3. Multiplexed Interfacial Transduction of Nucleic Acid Hybridization Using a Single Color of Immobilized Quantum Dot Donor and Two Acceptors in Fluorescence Resonance Energy Transfer [J] . W. Russ Algar, Ulrich J. Krull Analytical chemistry . 2010,第1期

机译：使用单色固定的量子点供体和两个受主在荧光共振能量转移中的核酸杂交的多重界面转导。
4. Probing the interaction between single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor [C] . Weiss, S., Ha, Quantum Electronics and Laser Science Conference, 1996. QELS '96., Summaries of Papers Presented at the . 2001

机译：探测单个分子之间的相互作用：单个供体和单个受体之间的荧光共振能量转移
5. Study of protein-RNA interactions using fluorescence resonance energy transfer (FRET) and single-molecule FRET. [D] . Lamichhane, Rajan. 2011

机译：使用荧光共振能量转移（FRET）和单分子FRET研究蛋白质-RNA相互作用。
6. Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor. [O] . T Ha, T Enderle, D F Ogletree, 1996

机译：探索两个单个分子之间的相互作用：单个供体和单个受体之间的荧光共振能量转移。
7. Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor. [O] . Ha, T, Enderle, T, Ogletree, D F, 1996

机译：探索两个单个分子之间的相互作用：单个供体和单个受体之间的荧光共振能量转移。
8. Materials for Fluorescence Resonance Energy Transfer Analysis: Beyond Traditional Donor-Acceptor Combinations. [R] . Sapsford, K. E., Berti, L., Medintz, I. L. 2006

机译：用于荧光共振能量转移分析的材料：超越传统的供体 - 受体组合。

Probing the interaction between two single molecules: Fluorescence resonance energy transfer between a single donor and a single acceptor

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