Molecular cloning of the first metazoan β-1,3 glucanase from eggs of the sea urchin Strongylocentrotus purpuratus

Eric Scott Bachman; David R. McClay

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Molecular cloning of the first metazoan β-1,3 glucanase from eggs of the sea urchin Strongylocentrotus purpuratus

机译：从海胆鸡蛋中的第一个后生动物β-1,3葡聚糖酶的分子克隆

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e report the molecular cloning of the first β-1,3 glucanase from animal tissue. Three peptide sequences were obtained from β-1,3 glucanase that had been purified from eggs of the sea urchin Strongylocentrotus purpuratus and the gene was cloned by PCR using oligonucleotides deduced from the peptide sequences. The full-length cDNA shows a predicted enzyme structure of 499 aa with a hydrophobic signal sequence. A 3.2-kb message is present in eggs, during early embryogenesis, and in adult gut tissue.

机译：e报告了动物组织中第一个β-1,3葡聚糖酶的分子克隆。从已经从海胆Strongylocentrotus purpuratus的卵中纯化的β-1，3葡聚糖酶获得了三个肽序列，并使用从该肽序列推导的寡核苷酸通过PCR克隆了该基因。全长cDNA显示具有疏水信号序列的499个氨基酸的预期酶结构。在卵子，早期胚胎发生和成年肠道组织中存在3.2kb的信息。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第13期|p.6808-6813|共6页
作者
Eric Scott Bachman; David R. McClay;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. beta-1,3-Glucanase from Unfertilized Eggs of the Sea Urchin Strongylocentrotus intermedius. Comparison with beta-1,3-Glucanases of Marine and Terrestrial Mollusks [J] . V.V. Sova, N. I. Shirokova, M. I. Kusaykin, Biochemistry . 2003,第5期

机译：β-1，3-葡聚糖酶来自中间的海胆Strongylocentrotus intermedius的卵。与海洋和陆地软体动物的β-1,3-葡聚糖酶的比较
2. Beta-1,3-glucanase from unfertilized eggs of sea urchin Strongylocentrotus intermedius. Comparison with beta-1,3-glucanases of marine and terrestrial mollusks [J] . V.V. Sova, N.I. Shirokova, M.I. Kusaykin, Биохимия . 2003,第5期

机译：来自海胆Strongylocentrotus intermedius的未受精卵的β-1,3-葡聚糖酶。与海洋和陆地软体动物的β-1,3-葡聚糖酶的比较
3. Beta-1,3-glucanase from unfertilized eggs of sea urchin Strongylocentrotus intermedius. Comparison with beta-1,3-glucanases of marine and terrestrial mollusks [J] . V.V. Sova, N.I. Shirokova, M.I. Kusaykin, Биохимия . 2003,第5期

机译：β-1,3-葡聚糖酶来自海胆强化卵子血清素骨膜中间核。与海洋和陆地软体动物的β-1,3-葡聚糖酶进行比较
4. Expression of Pigment Cell-Specific Genes in the Ontogenesis of the Sea Urchin Strongylocentrotus intermedius [C] . Natalya V. Ageenko, Konstantin V. Kiselev, Nelly A. Odintsova International Marine Biotechnology Conference . 2010

机译：色素细胞特异性基因在中间型海胆本体发育中的表达
5. Examining the evolution of phosphagen kinases: A study of a dimeric arginine kinase from sea urchin (Strongylocentrotus purpuratus) eggs [D] . Held, Brenda Christine 2007

机译：检查磷酸激酶的进化：海胆卵（Strongylocentrotus purpuratus）卵二聚精氨酸激酶的研究
6. Molecular cloning of the first metazoan beta-13 glucanase from eggs of the sea urchin Strongylocentrotus purpuratus. [O] . E S Bachman, D R McClay 1996

机译：从海胆Strongylocentrotus purpuratusus卵中的第一个后生动物β-13葡聚糖酶的分子克隆。
7. Molecular cloning of the first metazoan beta-1,3 glucanase from eggs of the sea urchin Strongylocentrotus purpuratus. [O] . Bachman, E S, McClay, D R 1996

机译：从海胆Strongylocentrotus purpuratusus卵中的第一个后生动物β-1,3葡聚糖酶的分子克隆。

Molecular cloning of the first metazoan β-1,3 glucanase from eggs of the sea urchin Strongylocentrotus purpuratus

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