Overview of the most prevalent hypothalamus-specific mRNAs, as identified by directional tag PCR subtraction

Kaare M. Gautvik; Luis de Lecea; Vigdis T. Gautvik

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Overview of the most prevalent hypothalamus-specific mRNAs, as identified by directional tag PCR subtraction

机译：通过方向标记PCR减法鉴定的最普遍的下丘脑特异性mRNA概述

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We applied the directional tag PCR subtrac- ive hybridization method to construct a rat hypothalamic cDNA library from which cerebellar and hippocampal se- uences had been depleted, enriching 20-30-fold for se- uences expressed selectively in the hypothalamus. We studied a sample of 94 clones selected for enrichment in the subtracted library. These clones corresponded to 43 distinct mRNA species, about half of which were novel.

机译：我们应用了定向标记PCR置换杂交法，构建了大鼠下丘脑cDNA文库，小脑和海马蛋白已经耗尽，下丘脑中选择性表达的蛋白丰富了20-30倍。我们研究了94个克隆的样本，这些克隆被选择用于在扣除的文库中进行富集。这些克隆对应于43种不同的mRNA种类，其中大约一半是新的。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第16期|p.8733-8738|共6页
作者
Kaare M. Gautvik; Luis de Lecea; Vigdis T. Gautvik;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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6. Overview of the most prevalent hypothalamus-specific mRNAs as identified by directional tag PCR subtraction. [O] . K M Gautvik, L de Lecea, V T Gautvik, 1996

机译：最流行的下丘脑特异的mRNA概述通过定向标记PCR减法鉴定。
7. Overview of the most prevalent hypothalamus-specific mRNAs, as identified by directional tag PCR subtraction. [O] . Gautvik, K M, de Lecea, L, Gautvik, V T, 1996

机译：最流行的下丘脑特异的mRNA概述，通过定向标记PCR减法鉴定。

Overview of the most prevalent hypothalamus-specific mRNAs, as identified by directional tag PCR subtraction

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