Structure and function in rhodopsin: Expression of functional mammalian opsin in Saccharomyces cerevisiae

Ramin Mollaaghababa; Florence F. Davidson; Chris Kaiser

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Structure and function in rhodopsin: Expression of functional mammalian opsin in Saccharomyces cerevisiae

机译：视紫红质的结构和功能：功能性哺乳动物视蛋白在酿酒酵母中的表达

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The yeast Saccharomyces cerevisiae has been investigated for expression of mammalian opsin as an alter- native to the currently used expression in COS-1 mammalian cells. The synthetic opsin gene was placed under the control of the inducible promoter GAL1 in the multicopy yeast/ Escherichia coli shuttle vector YEpRF1. Transformation of a GAL~+ S. cerevisiae strain with the vector and growth of galactose-induced cultures to saturation showed the produc- tion of 2.0 + - 0.5 mg of opsin from about 10~10 cells by ELISA.

机译：酵母酿酒酵母已被研究用于哺乳动物视蛋白的表达，这是目前在COS-1哺乳动物细胞中表达的一种替代形式。将合成的视蛋白基因置于多拷贝酵母/大肠杆菌穿梭载体YEpRF1中的诱导型启动子GAL1的控制下。用该载体转化GAL〜+啤酒酵母菌株，并使半乳糖诱导的培养物生长至饱和，通过ELISA从大约10〜10个细胞中产生了2.0±0.5 mg视蛋白。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第21期|p.11482-11486|共5页
作者
Ramin Mollaaghababa; Florence F. Davidson; Chris Kaiser;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Structure and function in rhodopsin: A tetracycline-inducible system in stable mammalian cell lines for high-level expression of opsin mutants [J] . Philip J. Reeves, Jong-Myoung Kim, H. Gobind Khorana Proceedings of the National Academy of Sciences of the United States of America . 2002,第21期

机译：视紫红质的结构和功能：稳定的哺乳动物细胞系中的四环素诱导系统，用于视蛋白突变体的高水平表达
2. Structure and function in rhodopsin: High level expression of a synthetic bovine opsin gene and its mutants in stable mammalian cell lines [J] . P. J. Reeves, R. L. Thurmond, H. G. Khorana Proceedings of the National Academy of Sciences of the United States of America . 1996,第21期

机译：在视紫红质中的结构和功能：合成的牛视蛋白基因及其突变体在稳定的哺乳动物细胞系中的高水平表达
3. Co-expression of a mammalian accessory trafficking protein enables functional expression of the rat MCT1 monocarboxylate transporter in Saccharomyces cerevisiae [J] . Makuc J, Cappellaro C, Boles E FEMS Yeast Research . 2004,第8期

机译：哺乳动物辅助运输蛋白的共表达使大鼠MCT1单羧酸盐转运蛋白在酿酒酵母中功能性表达
4. Functional Group Prediction of Un-annotated Protein by Exploiting Its Neighborhood Analysis in Saccharomyces Cerevisiae Protein Interaction Network [C] . Sovan Saha, Piyali Chatterjee, Subhadip Basu, International Doctoral Symposium on Applied Computation and Security Systems . 2017

机译：通过利用诸如酿酒酵母蛋白质相互作用网络的邻域分析来统计蛋白的功能群预测
5. Applying machine learning methods to suggest network involvement and functionality of genes in Saccharomyces cerevisiae [D] . Amthauer, Heather A. 2008

机译：应用机器学习方法建议酿酒酵母基因的网络参与和功能
6. Structure and function in rhodopsin: expression of functional mammalian opsin in Saccharomyces cerevisiae. [O] . R Mollaaghababa, F F Davidson, C Kaiser, 1996

机译：在视紫红质中的结构和功能：在酿酒酵母中表达功能性哺乳动物视蛋白。
7. Structure and function in rhodopsin: expression of functional mammalian opsin in Saccharomyces cerevisiae. [O] . Mollaaghababa, R, Davidson, F F, Kaiser, C, 1996

机译：在视紫红质中的结构和功能：在酿酒酵母中表达功能性哺乳动物视蛋白。
8. Expression of Mammalian Metallothionein Genes in Escherichia Coli and in Saccharomyces Cerevisiae [R] . Hou, Y. M. 1986

机译：哺乳动物金属硫蛋白基因在大肠杆菌和酿酒酵母中的表达

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Structure and function in rhodopsin: Expression of functional mammalian opsin in Saccharomyces cerevisiae

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