A novel cationic lipid greatly enhances plasmid DNA delivery and expression in mouse lung

Carl J. Wheeler; Philip L. Felgner; Yali J. Tsai

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A novel cationic lipid greatly enhances plasmid DNA delivery and expression in mouse lung

机译：一种新型阳离子脂质极大地增强了质粒DNA在小鼠肺中的传递和表达

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Effective gene therapy for lung tissue re- quires the use of efficient vehicles to deliver the gene of interest into lung cells. When plasmid DNA encoding chloramphenicol acetyltransferase (CAT) was administered intranasally to BALB/c mice without carrier lipids, CAT activity was detected in mouse lung extracts. Plasmid DNA delivered with optimally formulated commercially available transfection reagents ex- pressed up to 10-fold more CAT activity in lung than observed with naked DNA alone.

机译：对肺组织进行有效的基因治疗需要使用有效的载体将目的基因传递到肺细胞中。当向无载体脂质的BALB / c小鼠鼻内施用编码氯霉素乙酰转移酶（CAT）的质粒DNA时，在小鼠肺提取物中检测到CAT活性。用最佳配制的市售转染试剂递送的质粒DNA在肺中的CAT活性比仅裸DNA所观察到的高10倍。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第21期|p.11454-11459|共6页
作者
Carl J. Wheeler; Philip L. Felgner; Yali J. Tsai;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Transfection efficiency and toxicity following delivery of naked plasmid DNA and cationic lipid-DNA complexes to ovine lung segments. [J] . Emerson M, Renwick L, Tate S, Molecular therapy: the journal of the American Society of Gene Therapy . 2003,第4期

机译：将裸质粒DNA和阳离子脂质-DNA复合物递送至绵羊肺段后的转染效率和毒性。
2. Cationic lipids enhance cytokine and cell influx levels in the lung following administration of plasmid: cationic lipid complexes. [J] . Freimark BD, Blezinger HP, Florack VJ, The Journal of Immunology: Official Journal of the American Association of Immunologists . 1998,第9期

机译：施用质粒：阳离子脂质复合物后，阳离子脂质增强了肺中细胞因子和细胞内流的水平。
3. A new optimized formulation of cationic solid lipid nanoparticles intended for gene delivery: Development, characterization and DNA binding efficiency of TCERG1 expression plasmid [J] . FàbregasA., Sánchez-HernándezN., TicóJ.R., International Journal of Pharmaceutics . 2014,第1a2期

机译：用于基因传递的阳离子固体脂质纳米颗粒的新型优化配方：TCERG1表达质粒的开发，表征和DNA结合效率
4. Synthesis of Chrysin Based Cationic Lipids: Plasmid Delivery and Transgene Expression [C] . Bhavani Kedika, Venkatagiri Noole, Krishna Thotla, Applications of process engineering principles in materials processing, energy and environmental technologies . 2017

机译：基于Chrysin的阳离子脂质的合成：质粒传递和转基因表达
5. In Vitro Evaluation of Cationic Liposomal Delivery of BDNF Expressing Plasmid DNA in Experimental Model of Parkinson's Disease [D] . Avachat, Charul. 2017

机译：帕金森氏病实验模型中表达BDNF的质粒DNA阳离子脂质体递送的体外评估
6. A novel cationic lipid greatly enhances plasmid DNA delivery and expression in mouse lung. [O] . C J Wheeler, P L Felgner, Y J Tsai, 1996

机译：一种新型阳离子脂质极大地增强了质粒DNA在小鼠肺中的传递和表达。
7. A novel cationic lipid greatly enhances plasmid DNA delivery and expression in mouse lung. [O] . Wheeler, C J, Felgner, P L, Tsai, Y J, 1996

机译：一种新型阳离子脂质极大地增强了质粒DNA在小鼠肺中的传递和表达。
8. Cationic Lipid-Formulated Plasmid DNA Vaccine Confers Sustained Antibody-Mediated Protection Against Aerosolized Anthrax Spores [R] . Hermanson, G. , Whitlow, V. , Parker, S. , 2004

机译：阳离子脂质配方质粒DNa疫苗可提供持续的抗体介导保护，防止气雾化炭疽孢子

A novel cationic lipid greatly enhances plasmid DNA delivery and expression in mouse lung

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