Characterization of individual polynucleotide molecules using a membrane channel

John J. Kasianowicz; Eric Brandin; Daniel Branton

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Characterization of individual polynucleotide molecules using a membrane channel

机译：使用膜通道表征单个多核苷酸分子

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We show that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane. Because the channel diameter can accommodate only a single strand of RNA or DNA, each polymer traverses the membrane as an extended chain that partially blocks the channel. The passage of each molecule is detected as a transient decrease of ionic current whose duration is proportional to polymer length. Channel blockades can therefore be used to measure polynu- cleotide length. With further improvements, the method could in principle provide direct, high-speed detection of the se- quence of bases in single molecules of DNA or RNA.

机译：我们表明，电场可以驱动单链RNA和DNA分子通过脂质双层膜中的2.6 nm直径离子通道。由于通道直径只能容纳一条单链RNA或DNA，因此每种聚合物都以延伸链的形式横穿膜，从而部分阻塞了通道。每个分子的通过被检测为离子电流的瞬时减少，其持续时间与聚合物长度成正比。因此，通道阻滞可用于测量多核苷酸的长度。通过进一步的改进，该方法原则上可以直接，快速地检测DNA或RNA单个分子中碱基的序列。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1996年第24期|p.13770-13773|共4页
作者
John J. Kasianowicz; Eric Brandin; Daniel Branton;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip [J] . Louis P.Hromada, Brian J.Nablo, John J.Kasianowicz Lab on a chip . 2008,第4期

机译：使用基于聚合物的双层脂质膜芯片在单个膜结合离子通道内进行单分子测量
2. A MATHEMATICAL MODEL OF MEMBRANE GAS SEPARATION WITH ENERGY TRANSFER BY MOLECULES OF GAS FLOWING IN A CHANNEL TO MOLECULES PENETRATING THIS CHANNEL FROM THE ADJACENT CHANNEL [J] . Maciej Szwast, Zbigniew Szwast Chemical and Process Engineering Research . 2015,第2期

机译：气体从通道中流过的分子到相邻通道中穿透该通道的分子进行能量转移的膜气体分离的数学模型
3. A MATHEMATICAL MODEL OF MEMBRANE GAS SEPARATION WITH ENERGY TRANSFER BY MOLECULES OF GAS FLOWING IN A CHANNEL TO MOLECULES PENETRATING THIS CHANNEL FROM THE ADJACENT CHANNEL [J] . Szwast Maciej, Szwast Zbigniew Inzynieria Chemiczna i Procesowa . 2015,第2期

机译：气体从通道中流过的分子到相邻通道中穿透该通道的分子进行能量转移的膜气体分离的数学模型
4. Visualizing Individual Rhodopsin (a G Protein-Coupled Receptor) Molecules in Native Disk and Reconstituted Membranes via Atomic Force Microscopy [C] . Eugene J. Choi, Albert J. Jin, Shui-Lin Niu, NSTI(Nano Science and Technology Institute) Nanotechnology Conference and Trade Show(Nanotech 2004) . 2004

机译：通过原子力显微镜可视化天然盘和重组膜中的单个视紫红质（G蛋白偶联受体）分子
5. Identification and Characterization of Small Molecule Inhibitors of Polynucleotide Kinase 3'-Phosphatase [D] . Moatti, Nathalie 2012

机译：多核苷酸激酶3'-磷酸酶小分子抑制剂的鉴定与表征
6. Characterization of individual polynucleotide molecules using a membrane channel [O] . John J. Kasianowicz, Eric Brandin, Daniel Branton, 1996

机译：使用A表征单个多核苷酸分子膜通道
7. A Mathematical Model of Membrane Gas Separation with Energy Transfer by Molecules of Gas Flowing in a Channel to Molecules Penetrating this Channel from the Adjacent Channel [O] . Szwast Maciej, Szwast Zbigniew 2015

机译：气体分子在相邻通道中穿过该通道的分子通道能量转移膜气体分离的数学模型

Characterization of individual polynucleotide molecules using a membrane channel

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