...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification of a major I-Ek-restricted determinant of hen egg lysozyme: limitations of lymph node proliferation studies in defining immunodominance and crypticity.
【24h】

Identification of a major I-Ek-restricted determinant of hen egg lysozyme: limitations of lymph node proliferation studies in defining immunodominance and crypticity.

机译：鸡蛋卵溶菌酶的主要I-Ek限制性决定因素的鉴定：淋巴结增殖研究在定义免疫显性和隐秘性方面的局限性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have chemically analyzed the peptides presented by I-Ek molecules after processing of hen egg lysozyme (HEL) by a murine B-lymphoma line or by splenocytes. In both cases, the identified peptides were derived from a single region of HEL, containing the core residues 85-96 with heterogeneous N and C termini. This was a surprising result because this determinant had previously been described as cryptic--i.e., not presented after processing of intact HEL. Examination of the specificities of T hybridomas isolated after immunization with either HEL or 84-96 peptide (p84-96) provided an explanation for this controversy. Whereas hybridomas induced by immunization with HEL responded equally well to HEL and p84-96, those induced by peptide immunization showed a marked preference for p84-96 over intact HEL. In other words, hybridomas isolated after p84-96 immunization responded poorly to forms of the 84-96 determinant produced by natural processing, leading to the possible erroneous interpretation that 84-96 is a hidden determinant. We conclude that (i) p84-96 is efficiently presented on I-Ek molecules after processing of HEL, (ii) the explanation for the weak lymph node response to this epitope after immunization with HEL lies at the level of the T cell, not the antigen-presenting cell, and (iii) crypticity cannot be defined on the basis of T-cell proliferation studies alone.
机译：我们已经通过鼠B淋巴瘤细胞系或脾细胞对鸡蛋溶菌酶（HEL）进行处理后，对I-Ek分子呈递的肽进行了化学分析。在这两种情况下，鉴定出的肽均来自HEL的单个区域，其中包含带有异质N和C末端的核心残基85-96。这是一个令人惊讶的结果，因为该行列式先前已被描述为神秘的-即在处理完整的HEL之后未显示。 HEL或84-96肽（p84-96）免疫后分离的T杂交瘤的特异性检查为这一争议提供了解释。 HEL免疫诱导的杂交瘤对HEL和p84-96的反应同样好，而肽免疫诱导的杂交瘤对p84-96的表达明显优于完整HEL。换句话说，p84-96免疫后分离的杂交瘤对自然加工产生的84-96决定簇的反应较差，导致可能错误地认为84-96是隐藏的决定簇。我们得出的结论是：（i）在处理HEL之后，p84-96有效地存在于I-Ek分子上；（ii）用HEL免疫后，针对该表位的弱淋巴结反应的解释在于T细胞的水平，而不是（iii）隐秘性不能仅基于T细胞增殖研究来定义。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号