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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The retinoblastoma-susceptibility gene product binds directly to the human TATA-binding protein-associated factor TAFII250.
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The retinoblastoma-susceptibility gene product binds directly to the human TATA-binding protein-associated factor TAFII250.

机译：视网膜母细胞瘤敏感性基因产物直接与人TATA结合蛋白相关因子TAFII250结合。

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摘要

RB, the protein product of the retinoblastoma tumor-suppressor gene, regulates the activity of specific transcription factors. This regulation appears to be mediated either directly through interactions with specific transcription factors or through an alternative mechanism. Here we report that stimulation of Sp1-mediated transcription by RB is partially abrogated at the nonpermissive temperature in ts13 cells. These cells contain a temperature-sensitive mutation in the TATA-binding protein-associated factor TAFII250, first identified as the cell cycle regulatory protein CCG1. The stimulation of Sp1-mediated transcription by RB in ts13 cells at the nonpermissive temperature could be restored by the introduction of wild-type human TAFII250. Furthermore, we demonstrate that RB binds directly to hTAFII250 in vitro and in vivo. These results suggest that RB can confer transcriptional regulation and possibly cell cycle control and tumor suppression through an interaction with TFIID, in particular with TAFII250.
机译：RB是视网膜母细胞瘤肿瘤抑制基因的蛋白质产物，调节特定转录因子的活性。这种调节似乎是通过与特定转录因子的相互作用或通过替代机制直接介导的。在这里，我们报告说在ts13细胞中，在非许可温度下，RB抑制了Sp1介导的转录刺激。这些细胞在TATA结合蛋白相关因子TAFII250中包含一个温度敏感突变，该突变首先被鉴定为细胞周期调节蛋白CCG1。通过引入野生型人TAFII250，可以恢复ts13细胞中RB在Sp13介导的Sp1介导的转录刺激。此外，我们证明了RB在体外和体内直接与hTAFII250结合。这些结果表明，RB可以通过与TFIID，特别是与TAFII250的相互作用来赋予转录调控，并且可能赋予细胞周期控制和肿瘤抑制作用。

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