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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Immunogenicity of transgenic plant-derived hepatitis B surface antigen.
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Immunogenicity of transgenic plant-derived hepatitis B surface antigen.

机译：转基因植物来源的乙型肝炎表面抗原的免疫原性。

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摘要

The focus of the Children's Vaccine Initiative is to encourage the discovery of technology that will make vaccines more readily available to developing countries. Our strategy has been to genetically engineer plants so that they can be used as inexpensive alternatives to fermentation systems for production of subunit antigens. In this paper we report on the immunological response elicited in vivo by using recombinant hepatitis B surface antigen (rHBsAg) purified from transgenic tobacco leaves. The anti-hepatitis B response to the tobacco-derived rHBsAg was qualitatively similar to that obtained by immunizing mice with yeast-derived rHBsAg (commercial vaccine). Additionally, T cells obtained from mice primed with the tobacco-derived rHBsAg could be stimulated in vitro by the tobacco-derived rHBsAg, yeast-derived rHBsAg, and by a synthetic peptide that represents part of the a determinant located in the S region (139-147) of HBsAg. Further support for the integrity of the T-cell epitope of the tobacco-derived rHBsAg was obtained by testing the ability of the primed T cells to proliferate in vitro after stimulation with a monoclonal anti-idiotype and an anti-idiotype-derived peptide, both of which mimic the group-specific a determinant of HBsAg. In total, we have conclusively demonstrated that both B- and T-cell epitopes of HBsAg are preserved when the antigen is expressed in a transgenic plant.
机译：儿童疫苗倡议的重点是鼓励发现使发展中国家更容易获得疫苗的技术。我们的策略是对植物进行基因工程改造，以便可以将它们用作生产亚单位抗原的发酵系统的廉价替代品。在本文中，我们报道了使用从转基因烟草叶片中纯化的重组乙型肝炎表面抗原（rHBsAg）在体内引起的免疫反应。从质量上讲，对源自烟草的rHBsAg的抗乙型肝炎反应与通过对酵母衍生的rHBsAg（商业疫苗）进行免疫而获得。此外，可以通过烟草来源的rHBsAg，酵母来源的rHBsAg以及代表位于S区的决定簇的一部分的合成肽在体外刺激从用烟草来源的rHBsAg引发的小鼠获得的T细胞刺激。 -147）的HBsAg。通过测试用单克隆抗独特型和抗独特型衍生的肽刺激后引发的T细胞在体外增殖的能力，获得了对烟草衍生的rHBsAg T细胞表位完整性的进一步支持。其中模仿组特异性HBsAg的决定因素。总的来说，我们已最终证明，当抗原在转基因植物中表达时，HBsAg的B细胞和T细胞抗原决定簇均被保留。

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