Degradation of the cleaved leader peptide of thiolase by a peroxisomal proteinase.

Authier F; Bergeron JJ; Ou WJ; Rachubinski RA; Posner BI; Walton PA

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Degradation of the cleaved leader peptide of thiolase by a peroxisomal proteinase.

机译：过氧化物酶体蛋白酶降解硫解酶切割的前导肽。

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A peroxisomal location for insulin-degrading enzyme (IDE) has been defined by confocal immunofluorescence microscopy of stably transfected CHO cells overexpressing IDE and digitonin-permeabilization studies in normal nontransfected fibroblasts. The functional significance of IDE in degrading cleaved leader peptides of peroxisomal proteins targeted by the type II motif was evaluated with a synthetic peptide corresponding to the type II leader peptide of prethiolase. The peptide effectively competed for degradation and cross-linking of the high-affinity substrate 125I-labeled insulin to IDE. Direct proteolysis of the leader peptide of prethiolase was confirmed by HPLC; degradation was inhibited by immunodepletion with an antibody to IDE. Phylogenetic analysis of proteinases related to IDE revealed sequence similarity to mitochondrial processing peptidases.

机译：胰岛素降解酶（IDE）的过氧化物酶体位置已通过共转染的过表达IDE的稳定转染的CHO细胞和正常未转染的成纤维细胞中的洋地黄素透化研究进行了共聚焦免疫荧光显微镜检查。用与预硫解酶的II型前导肽相对应的合成肽评价IDE在降解被II型基序靶向的过氧化物酶体蛋白的裂解的前导肽中的功能意义。该肽有效竞争高亲和力底物125 I标记的胰岛素与IDE的降解和交联。 HPLC证实前硫解酶前导肽的直接蛋白水解。通过用抗IDE抗体进行的免疫耗竭抑制了病毒的降解。与IDE相关的蛋白酶的系统发生分析揭示了与线粒体加工肽酶的序列相似性。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1995年第9期|P.3859-3863|共5页
作者
Authier F; Bergeron JJ; Ou WJ; Rachubinski RA; Posner BI; Walton PA;
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作者单位

Department of Anatomy-Cell Biology, McGill University, Montreal, PQ Canada.;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
Acetyl CoA C Acetyltransferase metabolism; Endopeptidases metabolism; Microbodies; 微体;

机译：乙酰辅酶A乙酰转移酶代谢;内肽酶代谢;抗体;微体;

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5. Design and synthesis of novel cleavable detergents for protein and peptide analysis by mass spectrometry. [D] . Norris, Jeremy L. 2003

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6. Degradation of the cleaved leader peptide of thiolase by a peroxisomal proteinase. [O] . F Authier, J J Bergeron, W J Ou, 1995

机译：过氧化物酶体蛋白酶降解硫解酶切割的前导肽。
7. Degradation of the cleaved leader peptide of thiolase by a peroxisomal proteinase. [O] . Authier, F, Bergeron, J J, Ou, W J, 1995

机译：过氧化物酶体蛋白酶降解硫解酶切割的前导肽。

Degradation of the cleaved leader peptide of thiolase by a peroxisomal proteinase.

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