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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Electrical and synaptic properties of embryonic luteinizing hormone-releasing hormone neurons in explant cultures.
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Electrical and synaptic properties of embryonic luteinizing hormone-releasing hormone neurons in explant cultures.

机译：胚胎黄体生成素释放激素神经元在外植体培养物中的电和突触特性。

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摘要

Voltage- and ligand-activated channels in embryonic neurons containing luteinizing hormone-releasing hormone (LHRH) were studied by patch-pipette, whole-cell current and voltage clamp techniques. LHRH neurons were maintained in explant cultures derived from olfactory pit regions of embryonic mice. Cells were marked intracellularly with Lucifer yellow following recording. Sixty-two cells were unequivocally identified as LHRH neurons by Lucifer yellow and LHRH immunocytochemistry. The cultured LHRH neurons had resting potentials around -50 mV, exhibited spontaneous discharges generated by intrinsic and/or synaptic activities and contained a time-dependent inward rectifier (Iir). Voltage clamp analysis of ionic currents in the LHRH neuron soma revealed a tetrodotoxin-sensitive Na+ current (INa) and two major types of K+ currents, a transient current (IA), a delayed rectifier current (IK) and low- and high-voltage-activated Ca2+ currents. Spontaneous depolarizing synaptic potentials and depolarizations induced by direct application of gamma-aminobutyrate were both inhibited by picrotoxin or bicuculline, demonstrating the presence of functional gamma-aminobutyrate type A synapses on these neurons. Responses to glutamate were found in LHRH neurons in older cultures. Thus, embryonic LHRH neurons not yet positioned in their postnatal environment in the forebrain contained a highly differentiated repertoire of voltage- and ligand-gated channels.
机译：通过贴片移液管，全细胞电流和电压钳技术研究了含有黄体生成素释放激素（LHRH）的胚胎神经元中的电压和配体激活通道。 LHRH神经元保持在源自胚胎小鼠嗅觉凹坑区域的外植体培养物中。记录后，细胞在细胞内用路西法黄标记。通过路西法黄和LHRH免疫细胞化学法明确地将62个细胞鉴定为LHRH神经元。培养的LHRH神经元的静息电位为-50 mV，表现出由内在和/或突触活动产生的自发放电，并包含时间依赖性向内整流器（Iir）。对LHRH神经元体中离子电流的电压钳位分析显示了对河豚毒素敏感的Na +电流（INa）和两种主要的K +电流，即瞬态电流（IA），延迟整流器电流（IK）和低压和高压激活的Ca2 +电流。微小毒素或双小分子抑制了自然产生的自发去极化突触电位和直接施加的γ-氨基丁酸酯诱导的去极化，表明这些神经元上存在功能性γ-氨基丁酸酯A型突触。在较老的文化中，LHRH神经元中发现了对谷氨酸的反应。因此，尚未定位在前脑的产后环境中的胚胎LHRH神经元包含高度分化的电压门控和配体门控通道。

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