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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Role of sodium and potassium ions in regulation of glucose metabolism in cultured astroglia.
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Role of sodium and potassium ions in regulation of glucose metabolism in cultured astroglia.

机译：钠和钾离子在培养的星形胶质细胞中葡萄糖代谢调节中的作用。

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摘要

Effects of increasing extracellular K+ or intracellular Na+ concentrations on glucose metabolism in cultures of rat astroglia and neurons were examined. Cells were incubated in bicarbonate buffer, pH 7.2, containing 2 mM glucose, tracer amounts of [14C]deoxyglucose ([14C]dGlc), and 5.4, 28, or 56 mM KCl for 10, 15, or 30 min, and then for 5 min in [14C]dGlc-free buffer to allow efflux of unmetabolized [14C]dGlc. Cells were then digested and assayed for labeled products, which were shown to consist of 96-98% [14C]deoxyglucose 6-phosphate. Increased K+ concentrations significantly raised [14C]deoxyglucose 6-phosphate accumulation in both neuronal and mixed neuronal-astroglial cultures at 15 and 30 min but did not raise it in astroglial cultures. Veratridine (75 microM), which opens voltage-dependent Na+ channels, significantly raised rates of [14C]dGlc phosphorylation in astroglial cultures (+20%), and these elevations were blocked by either 1 mM ouabain, a specific inhibitor of Na+,K(+)-ATPase (EC 3.6.1.37), or 10 microM tetrodotoxin, which blocks Na+ channels. The carboxylic sodium ionophore, monensin (10 microM), more than doubled [14C]dGlc phosphorylation; this effect was only partially blocked by ouabain and unaffected by tetrodotoxin. L-Glutamate (500 microM) also stimulated [14C]dGlc phosphorylation in astroglia--not through N-methyl-D-aspartate or non-N-methyl-D-aspartate receptor mechanisms but via a Na(+)-dependent glutamate-uptake system. These results indicate that increased uptake of Na+ can stimulate energy metabolism in astroglial cells.
机译：研究了增加的细胞外K +或细胞内Na +浓度对大鼠星形胶质细胞和神经元培养物中葡萄糖代谢的影响。将细胞在含有2 mM葡萄糖，示踪量的[14C]脱氧葡萄糖（[14C] dGlc）和5.4、28或56 mM KCl的碳酸氢盐缓冲液（pH 7.2）中孵育10、15或30分钟，然后在不含[14C] dGlc的缓冲液中放置5分钟，以使未代谢的[14C] dGlc流出。然后消化细胞并分析标记产物，显示其由96-98％[14C]脱氧葡萄糖6-磷酸组成。增加的K +浓度会在15和30分钟时显着提高神经元和混合神经元-星形胶质细胞培养物中[14C]脱氧葡萄糖6-磷酸的积累，但在星形胶质细胞培养物中却没有升高。 Veratridine（75 microM），其打开了电压依赖性Na +通道，显着提高了星形胶质细胞培养物中[14C] dGlc磷酸化的速率（+ 20％），并且这些升高均被1 mM ouabain（Na +，K的特异性抑制剂）阻止（+）-ATPase（EC 3.6.1.37）或10 microM河豚毒素，可阻断Na +通道。羧基钠离子载体莫能菌素（10 microM），使[14C] dGlc磷酸化增加了一倍以上；这种作用仅被哇巴因部分阻断，而不受河豚毒素的影响。 L-谷氨酸（500 microM）还刺激了星形胶质细胞中的[14C] dGlc磷酸化-不是通过N-甲基-D-天冬氨酸或非N-甲基-D-天冬氨酸受体机制，而是通过Na（+）依赖性谷氨酸-吸收系统。这些结果表明，Na +吸收的增加可以刺激星形胶质细胞的能量代谢。

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