...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Estrogen regulates the expression of several different estrogen receptor mRNA isoforms in rat pituitary.
【24h】

Estrogen regulates the expression of several different estrogen receptor mRNA isoforms in rat pituitary.

机译：雌激素调节大鼠垂体中几种不同的雌激素受体mRNA亚型的表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A 5.2-kb mRNA band that contains estrogen receptor (ER) sequence and exhibits sex- and tissue-specific expression has been identified in rat pituitary via Northern analysis; this band is composed of at least two distinctive ER mRNA isoforms. This mRNA is expressed in high levels in female pituitary but is absent in male pituitary and uterus, whereas the mRNA encoding the full-length receptor (6.2 kb) is expressed in all the aforementioned tissues. Estradiol treatment potently induces the expression of the 5.2-kb band in the male pituitary. Oligonucleotide hybridization and ribonuclease-protection experiments indicate that the pituitary ER variant is missing exons 1-4. Two corresponding cDNA clones, truncated estrogen receptor product 1 and 2 (TERP-1 and TERP-2), were isolated by using the anchored PCR. Both sequences contain a 31-bp segment of specific sequence upstream of exon 5; TERP-2, however, contains an additional 66 bp of specific sequence between the 31-bp segment and exon 5. On Northern analysis, probes complementary to the 31-bp segment of specific sequence hybridize only to the 5.2-kb band. Immunoblotting identified several proteins in rat pituitary that could represent the translation products of these or related transcripts. In summary, several ER isoforms have been identified that exhibit both tissue-specific expression and marked estrogen regulation and differ from full-length receptor by virtue of sequence upstream of the exon 4/5 boundary. Physiologically, the putative proteins encoded by these or similar isoforms might be important modulators of the tissue- and promoter-specific effects of estradiol.
机译：通过Northern分析已在大鼠垂体中鉴定出一条含有雌激素受体（ER）序列并表现出性别和组织特异性表达的5.2-kb mRNA带。该条带由至少两个独特的ER mRNA同工型组成。该mRNA在雌性垂体中高水平表达，但在雄性垂体和子宫中不存在，而编码全长受体（6.2kb）的mRNA在所有上述组织中表达。雌二醇治疗有效地诱导了男性垂体中5.2-kb带的表达。寡核苷酸杂交和核糖核酸酶保护实验表明垂体ER变体缺少第1-4外显子。使用锚定PCR分离了两个相应的cDNA克隆，即截短的雌激素受体产物1和2（TERP-1和TERP-2）。这两个序列在​​外显子5的上游均含有一个特定序列的31 bp片段；但是，TERP-2在31 bp片段和外显子5之间包含一个额外的66 bp特异性序列。在Northern分析中，与该特定序列31 bp片段互补的探针仅与5.2 KB波段杂交。免疫印迹鉴定了大鼠垂体中的几种蛋白质，它们可以代表这些或相关转录本的翻译产物。总之，已经鉴定出几种ER同工型，其表现出组织特异性表达和显着的雌激素调节，并且由于外显子4/5边界上游的序列而不同于全长受体。从生理上讲，由这些或相似亚型编码的推定蛋白质可能是雌二醇的组织和启动子特异性作用的重要调节剂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号