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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Arabidopsis COP1 protein specifically interacts in vitro with a cytoskeleton-associated protein, CIP1.
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Arabidopsis COP1 protein specifically interacts in vitro with a cytoskeleton-associated protein, CIP1.

机译：拟南芥COP1蛋白在体外与细胞骨架相关蛋白CIP1特异性相互作用。

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摘要

Arabidopsis COP1 acts inside the nucleus to suppress photomorphogenic cellular development, and light inactivation of COP1 may involve a specific control of its nuclear activity in hypocotyls and cotyledons, but not in roots, of developing seedlings. To understand the molecular mechanisms of COP1 action during light-mediated development, we initiated a screen for Arabidopsis cDNAs encoding proteins which interact directly with COP1 in vitro as a step to identify the cellular components involved. We report here the isolation and characterization of a cDNA clone encoding a protein designated CIP1 (COP1-interactive protein 1). CIP1 is predominantly alpha-helical and most likely involved in coiled-coil formation. It interacts specifically with the putative coiled-coil region of COP1 in vitro. Further, CIP1 is encoded by a single gene in Arabidopsis, and its mRNA and protein levels are not regulated by light. Immunofluorescent labeling of CIP1 in Arabidopsis seedling protoplasts demonstrated that CIP1 is part of, or associated with, a cytoskeletal structure in hypocotyl and cotyledon cells, but not in roots. Our results are consistent with a possible role of CIP1 in mediating light control of COP1 nuclear activity by regulating its nucleocytoplasmic partitioning.
机译：拟南芥COP1在细胞核内起作用，以抑制光形态发生细胞的发育，并且COP1的光失活可能涉及发育中幼苗的下胚轴和子叶中而不是根中对其核活性的特定控制。为了了解在光介导的发育过程中COP1作用的分子机制，我们启动了筛选拟南芥cDNA编码蛋白的筛选工作，该蛋白可直接与COP1在体外相互作用，以此作为鉴定所涉及的细胞成分的步骤。我们在这里报告编码称为CIP1（COP1互动蛋白1）的蛋白质的cDNA克隆的分离和表征。 CIP1主要为α-螺旋，最有可能参与卷曲螺旋的形成。它在体外与COP1的推定螺旋线圈区域特异性相互作用。此外，CIP1由拟南芥中的单个基因编码，其mRNA和蛋白质水平不受光照调节。拟南芥幼苗原生质体中CIP1的免疫荧光标记表明CIP1是下胚轴和子叶细胞中细胞骨架结构的一部分或与之相关，但在根中却不存在。我们的结果与CIP1在调节COP1核细胞质分区中介导COP1核活性的光控制中可能发挥的作用相一致。

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