Catalytically critical nucleotide in domain 5 of a group II intron.

Peebles CL; Zhang M; Perlman PS; Franzen JS

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Catalytically critical nucleotide in domain 5 of a group II intron.

机译：II组内含子的结构域5中的催化关键核苷酸。

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摘要

Domain 5 (D5) is a small hairpin structure within group II introns. A bimolecular assay system depends on binding by D5 to an intron substrate for self-splicing activity. In this study, mutations in D5 identify two among six nearly invariant nucleotides as being critical for 5' splice junction hydrolysis but unimportant for binding. A mutation at another site in D5 blocks binding. Thus, mutations can distinguish two D5 functions: substrate binding and catalysis. The secondary structure of D5 may resemble helix I formed by the U2 and U6 small nuclear RNAs in the eukaryotic spliceosome. Our results support a revision of the previously proposed correspondence between D5 and helix I on the basis of the critical trinucleotide 5'-AGC-3' present in both. We suggest that this trinucleotide plays a similar role in promoting the chemical reactions for both splicing systems.

机译：域5（D5）是II组内含子中的一个小发夹结构。双分子测定系统依靠D5与内含子底物的结合来实现自我剪接活性。在这项研究中，D5中的突变确定了6个几乎不变的核苷酸中的2个对5'剪接连接水解至关重要，但对结合而言并不重要。 D5中另一个位点的突变会阻止结合。因此，突变可以区分两个D5功能：底物结合和催化。 D5的二级结构可能类似于由真核剪接体中的U2和U6小核RNA形成的螺旋I。我们的结果支持基于之前提出的D5和螺旋I之间存在的关键三核苷酸5'-AGC-3'的对应关系的修订。我们建议这种三核苷酸在促进两个剪接系统的化学反应中起相似的作用。

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来源
《Proceedings of the National Academy of Sciences of the United States of America》 |1995年第10期|P.4422-4426|共5页
作者
Peebles CL; Zhang M; Perlman PS; Franzen JS;
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作者单位

Department of Biological Sciences, University of Pittsburgh, PA 15260-7700, USA.;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
Introns; Nucleic Acid Conformation; RNA; RNA Precursors; bacteriophage T7-induced RNA polymerase; 内含子; 核酸构象;

机译：Introns;Nucleic Acid Conformation;RNA;RNA Precursors;bacteriophage T7-induced RNA polymerase;内含子;核酸构象;

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专利

1. Studies of point mutants define three essential paired nucleotides in the domain 5 substructure of a group II intron. [J] . S C Boulanger, S M Belcher, U Schmidt, Molecular and Cellular Biology . 1995,第8期

机译：点突变体的研究在II组内含子的结构域5子结构中定义了三个必需的配对核苷酸。
2. Photocrosslinking of 4-thio uracil-containing RNAs supports a side-by-side arrangement of domains 5 and 6 of a group II intron. [J] . Podar M, Perlman PS RNA . 1999,第2期

机译：含4-硫尿嘧啶的RNA的光交联支持II组内含子的结构域5和6的并排排列。
3. Critical Contacts between the Eukaryotic Initiation Factor 2B (eIF2B) Catalytic Domain and both eIF2β and -2γ Mediate Guanine Nucleotide Exchange [J] . Sarah S. Mohammad-Qureshi, Rapha?l Haddad, Elizabeth J. Hemingway, Molecular and Cellular Biology . 2007,第14期

机译：真核起始因子2B（eIF2B）催化域与eIF2β和-2γ两者之间的关键接触介导鸟嘌呤核苷酸交换。
4. The 3D structure of DNA-free EndoVIII suggests inter-domain conformational changes of catalytic significance [C] . Gali Golan, Dmitry O.Zharkov, Hadar Feinberg International Peptide Symposium;European Peptide Symposium . 2005

机译：DNA-FeeRoviii的3D结构表明催化意义的域间构象变化
5. Investigation of an unusual metal -RNA cluster in the P5abc subdomain of the group I intron. [D] . Burns, Shannon Naomi. 2004

机译：第一组内含子的P5abc子域中一个不寻常的金属-RNA簇的研究。
6. Catalytically critical nucleotide in domain 5 of a group II intron. [O] . C L Peebles, M Zhang, P S Perlman, 1995

机译：II组内含子的结构域5中的催化关键核苷酸。
7. Catalytically critical nucleotide in domain 5 of a group II intron. [O] . Peebles, C L, Zhang, M, Perlman, P S, 1995

机译：II组内含子的结构域5中的催化关键核苷酸。

Catalytically critical nucleotide in domain 5 of a group II intron.

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