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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Induction of ubiquitin-conjugating enzymes during terminal erythroid differentiation.
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Induction of ubiquitin-conjugating enzymes during terminal erythroid differentiation.

机译：在终末红系分化过程中诱导泛素结合酶。

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A global cellular reorganization occurs during the reticulocyte stage of erythroid differentiation. This reorganization is accomplished partly through programmed protein degradation. The selection of proteins for degradation can be mediated by covalent attachment of ubiquitin. We have cloned cDNAs encoding two ubiquitin-conjugating (E2) enzymes, E2-20K and E2-230K, and found their genes to be strongly induced during the differentiation of erythroblasts into reticulocytes. Induction of the E2-20K and E2-230K genes is specific, as transcript levels for at least two other ubiquitinating enzymes fall during erythroblast differentiation. In contrast to most proteins induced in reticulocytes, E2-20K and E2-230K enzymes are present at strongly reduced levels in erythrocytes and thus decline in abundance as reticulocyte maturation is completed. This result suggests that both enzymes function during the reticulocyte stage, when enhanced protein degradation has been observed. These data implicate regulated components of the ubiquitin conjugation machinery in erythroid differentiation.
机译：在红系分化的网织红细胞阶段发生全局细胞重组。这种重组部分是通过程序化的蛋白质降解来完成的。降解蛋白的选择可以通过泛素的共价连接来介导。我们已经克隆了编码两种泛素结合（E2）酶E2-20K和E2-230K的cDNA，发现它们的基因在成红细胞分化为网状细胞的过程中被强烈诱导。 E2-20K和E2-230K基因的诱导是特异性的，因为至少两种其他泛素化酶的转录水平在成红细胞分化过程中下降。与网状细胞中诱导的大多数蛋白质相反，E2-20K和E2-230K酶在红细胞中的含量大大降低，因此随着网状细胞成熟的完成，丰度下降。该结果表明，当观察到增强的蛋白质降解时，两种酶都在网织红细胞阶段起作用。这些数据暗示了泛素结合机制在红系分化中的调控成分。

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