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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Human steroidogenic acute regulatory protein: functional activity in COS-1 cells, tissue-specific expression, and mapping of the structural gene to 8p11.2 and a pseudogene to chromosome 13.

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Human steroidogenic acute regulatory protein: functional activity in COS-1 cells, tissue-specific expression, and mapping of the structural gene to 8p11.2 and a pseudogene to chromosome 13.

机译：人类类固醇生成性急性调节蛋白：COS-1细胞中的功能活性，组织特异性表达以及结构基因到8p11.2和假基因到13号染色体的映射。

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摘要

Steroidogenic acute regulatory protein (StAR) appears to mediate the rapid increase in pregnenolone synthesis stimulated by tropic hormones. cDNAs encoding StAR were isolated from a human adrenal cortex library. Human StAR, coexpressed in COS-1 cells with cytochrome P450scc and adrenodoxin, increased pregnenolone synthesis > 4-fold. A major StAR transcript of 1.6 kb and less abundant transcripts of 4.4 and 7.5 kb were detected in ovary and testis. Kidney had a lower amount of the 1.6-kb message. StAR mRNA was not detected in other tissues including placenta. Treatment of granulosa cells with 8-bromo-adenosine 3',5'-cyclic monophosphate for 24 hr increased StAR mRNA 3-fold or more. The structural gene encoding StAR was mapped using somatic cell hybrid mapping panels to chromosome 8p. Fluorescence in situ hybridization placed the StAR locus in the region 8p11.2. A StAR pseudogene was mapped to chromosome 13. We conclude that StAR expression is restricted to tissues that carry out mitochondrial sterol oxidations subject to acute regulation by cAMP and that StAR mRNA levels are regulated by cAMP.

机译：类固醇激素急性调节蛋白（StAR）似乎介导了由热带激素刺激的孕烯醇酮合成的快速增加。从人类肾上腺皮质文库中分离出编码StAR的cDNA。人StAR与细胞色素P450scc和肾上腺毒素共表达于COS-1细胞中，孕烯醇酮合成增加> 4倍。在卵巢和睾丸中检测到主要的StAR转录物为1.6 kb，而较少的转录物为4.4和7.5 kb。肾脏的1.6 kb消息量较小。在包括胎盘在内的其他组织中未检测到StAR mRNA。用8-溴腺苷3'，5'-环一磷酸处理颗粒细胞24小时可使StAR mRNA增长3倍或更多。使用体细胞杂种作图小组将编码StAR的结构基因作图到染色体8p。荧光原位杂交将StAR基因座置于8p11.2区域。一个StAR假基因被定位到13号染色体。我们得出的结论是，StAR的表达仅限于进行线粒体固醇氧化的组织，该组织受cAMP的急性调节，而StAR mRNA的水平受cAMP的调节。

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来源
《Proceedings of the National Academy of Sciences of the United States of America》 |1995年第11期|P.4778-4782|共5页
作者
Sugawara T; Holt JA; Driscoll D; Strauss JF 3rd; Lin D; Miller WL; Patterson D; Clancy KP; Hart IM; Clark BJ; al.;
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作者单位

Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia 19104, USA.;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
Chromosomes; Human; Pair 13; Chromosomes; Human; Pair 8; Gene Expression; 染色体; 人; 13对; 染色体; 人; 8对; 基因表达; 基因; 结构; 磷蛋白类; 拟基因;

机译：Chromosomes;Human;Pair 13;Chromosomes;Human;Pair 8;Gene Expression;染色体;人;13对;染色体;人;8对;基因表达;基因;结构;磷蛋白类;拟基因;

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1. Role of dosage-sensitive sex reversal, adrenal hypoplasia congenita, critical region on the X chromosome, gene 1 in protein kinase A- and protein kinase C-mediated regulation of the steroidogenic acute regulatory protein expression in mouse Leydig Tumor Cells: Mechanism of Action [J] . Manna PR, Dyson MT, Jo Y, Endocrinology . 2009,第1期

机译：剂量敏感性性逆转，先天性肾上腺皮质发育不全，X染色体上的关键区域，基因1在小鼠Leydig肿瘤细胞中类固醇生成的急性调节蛋白表达的蛋白激酶A和蛋白激酶C介导的调节中的作用
2. Steroidogenic acute regulatory protein gene expression, steroid-hormone secretion and proliferative activity of adrenocortical cells in the presence of proteasome inhibitors: In vivo studies on the regenerating rat adrenal cortex. [J] . Rucinski M, Tortorella C, Ziolkowska A, International journal of molecular medicine . 2008,第5期

机译：在蛋白酶体抑制剂存在下，类固醇激素急性调节蛋白基因表达，类固醇激素分泌和肾上腺皮质细胞的增殖活性：对再生大鼠肾上腺皮质的体内研究。
3. Rapid communication: mapping of the Steroidogenic Acute Regulatory Protein (StAR) gene to porcine chromosome 15 by linkage analysis using a novel PCR-RFLP. [J] . Allan?M F, Arango?J A, Hallock?J W, Journal of animal science . 1998,第2期

机译：快速交流：通过使用新型PCR-RFLP的连锁分析，将类固醇急性调节蛋白（StAR）基因定位到猪15号染色体。
4. Expression of Recombinant Human Bone Morphogenetic Protein 2 in Insect Cells, Purification and Activity Analysis [C] . Wen Chu, Shenheng Luo, Lihong Xu, International conference on applied biotechnology . 2014

机译：重组人骨形态发生蛋白2在昆虫细胞中的表达，纯化及活性分析
5. Molecular mechanisms for angiotensin II-dependent transcriptional activation of the human steroidogenic acute regulatory (StAR) protein gene [D] . Meier, Renate K. 2009

机译：人类类固醇生成急性调节（StAR）蛋白基因的血管紧张素II依赖性转录激活的分子机制
6. Human steroidogenic acute regulatory protein: functional activity in COS-1 cells tissue-specific expression and mapping of the structural gene to 8p11.2 and a pseudogene to chromosome 13. [O] . T Sugawara, J A Holt, D Driscoll, 1995

机译：人类类固醇生成性急性调节蛋白：COS-1细胞中的功能活性组织特异性表达以及结构基因到8p11.2和假基因到13号染色体的映射。
7. Human steroidogenic acute regulatory protein: functional activity in COS-1 cells, tissue-specific expression, and mapping of the structural gene to 8p11.2 and a pseudogene to chromosome 13. [O] . Sugawara, T, Holt, J A, Driscoll, D, 1995

机译：人类类固醇生成的急性调节蛋白：COS-1细胞中的功能活性，组织特异性表达以及结构基因到8p11.2和假基因到13号染色体的映射。

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